NDLI: Simplified analysis method of cell-free layers in blood flows as tool for the optimization of gas exchange devices

Content Provider	Springer Nature Link
Author	Rieper, Tina Čvančara, Paul Müller, Claas Reinecke, Holger
Copyright Year	2014
Abstract	A novel analyzing method is presented, which allows precise characterization of cell-free layers (CFLs) of blood flowing through microchannels. The CFL occurs due to axial migration of the erythrocytes (RBCs). A confocal laser scanning microscope (CLSM) is used to detect the reflected light of channel walls and cells within the blood flow. Since the presented method does not depend on emitted fluorescence signals, there is no necessity for a complex sample preparation as fluorescence marking of cells. Furthermore, it allows the characterization of the thickness of the CFL in whole blood. Due to the high vertical resolution of the used CLSM, the developed characterization method enables measurements along the optical axis of the microscope. It is exemplarily used to analyze the thickness of the CFL in human blood flowing through microchannels as a function of the hematocrit and blood flow velocity. The microchannels are made of silicone rubber with a height of 100 µm. The microchannels are intended for a gas exchange application.
Starting Page	1071
Ending Page	1078
Page Count	8
File Format	PDF
ISSN	16134982
Journal	Microfluidics and Nanofluidics
Volume Number	17
Issue Number	6
e-ISSN	16134990
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-04-05
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cell-free layer Blood flow Microchannels Confocal laser scanning microscope Engineering Fluid Dynamics Biomedical Engineering Analytical Chemistry Nanotechnology and Microengineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Materials Chemistry Condensed Matter Physics Electronic, Optical and Magnetic Materials

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