NDLI: L-Malic acid production within a microreactor with surface immobilised fumarase

Content Provider	Springer Nature Link
Author	Stojkovič, Gorazd Plazl, Igor Žnidaršič Plazl, Polona
Copyright Year	2010
Abstract	In recent years, microreactors have evolved from a technical novelty to a useful tool in many scientific laboratories. On the other hand, their implementation in industry proceeds with a slower pace. In this work, we present a microreactor system for continuous l-malic acid production that demonstrates potentials of microfluidic systems for production of this widely used organic acid. APTES and glutaraldehyde were used to covalently immobilise fumarase on glass microreactor walls in order to allow product–catalyst separation. The system was tested at different substrate concentrations and flow rates and conversion up to 80% could be obtained in appropriate conditions. The reaction was precisely predicted by the developed mathematical model. Kinetic studies were performed with both free and immobilised enzyme and the later was found to retain approximately 25% of free enzyme activity and had the activity half-life of 9 days.
Starting Page	627
Ending Page	635
Page Count	9
File Format	PDF
ISSN	16134982
Journal	Microfluidics and Nanofluidics
Volume Number	10
Issue Number	3
e-ISSN	16134990
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-10-10
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	l-Malic acid Microreactor Fumarase Immobilisation Mathematical model Engineering Fluid Dynamics Biomedical Engineering Industrial Chemistry/Chemical Engineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Materials Chemistry Condensed Matter Physics Electronic, Optical and Magnetic Materials

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