NDLI: A microfluidic device for studying the production of reactive oxygen species and the migration in lung cancer cells under single or coexisting chemical/electrical stimulation

Content Provider	Springer Nature Link
Author	Lo, Kai Yin Wu, Shang Ying Sun, Yung Shin
Copyright Year	2016
Abstract	Reactive oxygen species (ROS) are known to play an important role in the development of cancer, and many exogenous sources are believed to be related to the formation of ROS. For example, electric field is one of such factors reported to stimulate the production of ROS. Moreover, electric field is also shown to induce cell migration, a phenomenon termed electrotaxis. In this paper, a microfluidic chip was developed for studying the production of ROS and the migration in lung cancer cells under single or coexisting chemical/electrical stimulation. This chip has two unique features: (1) Five relative concentrations of 0, 1/8, 1/2, 7/8, and 1 are achieved in the culture regions; (2) five different strengths of EFs are produced inside these culture areas. Lung cancer cells were seeded inside this biocompatible chip for investigating their response to different concentrations of H2O2, a chemical stimulus known to increase the production of ROS. Then, the effects of honokiol, a chemical stimulus, in combination with electric field, a physical stimulus, on lung cancer cells were examined. Finally, lung cancer cell migration was investigated under single or combined honokiol/electric field treatments. The current microfluidic chip provides an in vitro platform mimicking the physiological condition where cells are under circulating conditions and are subject to controllable chemical/physical stimuli.
Starting Page	1
Ending Page	11
Page Count	11
File Format	PDF
ISSN	16134982
Journal	Microfluidics and Nanofluidics
Volume Number	20
Issue Number	1
e-ISSN	16134990
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-01-06
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Microfluidics Reactive oxygen species (ROS) Cell migration Electrotaxis Engineering Fluid Dynamics Biomedical Engineering Analytical Chemistry Nanotechnology and Microengineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Materials Chemistry Condensed Matter Physics Electronic, Optical and Magnetic Materials

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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