NDLI: Evaluation of the Temperature Responsive Stationary Phase Poly(N-isopropylacrylamide) in Aqueous LC for the Analysis of Small Molecules

Content Provider	Springer Nature Link
Author	Lynen, F. Heijl, J. M. D. Prez, F. E. Du Brown, R. Szucs, R. Sandra, P.
Copyright Year	2007
Abstract	Liquid chromatography columns were packed with a temperature responsive stationary phase based on poly(N-isopropylacrylamide) (PNIPAAm) attached to aminopropyl silicagel. This polymer shows hydrophilic properties below 32 °C and becomes hydrophobic above that temperature. The temperature responsive properties of the coupled phase are demonstrated using only water as mobile phase, whereby an increase in retention is observed with raising temperature. Mixtures of compounds covering a wide polarity range and including phenones, alkylbenzenes, phenols, alkylated benzoic acids, anilines, sulfonamides and carbamates were analyzed and the retention, peak shapes and plate counts were compared under identical conditions. For retained solutes, an increase in retention as a function of the temperature between 25 and 55 °C could be noted, whereby this was higher for the analytes containing a longer hydrophobic chain. Compounds with similar hydrophobic chains, but containing additional polar functions showed increased retention and improved peak shapes, suggesting a mixed mode interaction mechanism also at temperatures well above the transition temperature of the polymer. Weak acids and bases could be analyzed by pH adjustment. This is demonstrated for mixtures of benzoic acid derivatives and sulfamide drugs. A carbamate pesticide mixture was analyzed at 55 °C with water (pH 5.5) as mobile phase and ESI-MS detection. Temperature responsive stationary phases open perspectives for green chromatography.
Starting Page	143
Ending Page	150
Page Count	8
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	66
Issue Number	3-4
e-ISSN	16121112
Language	English
Publisher	Vieweg Verlag
Publisher Date	2007-06-19
Publisher Place	Wiesbaden
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Column liquid chromatography Aqueous liquid chromatography Temperature responsive stationary phase Small molecules Retention mechanism Measurement Science, Instrumentation Plant Sciences Biochemistry Pharmacy Organic Chemistry Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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