NDLI: Study of the Behavior of Water-Soluble Vitamins in HILIC on a Diol Column

Content Provider	Springer Nature Link
Author	Karatapanis, Andreas E. Fiamegos, Yiannis C. Stalikas, Constantine D.
Copyright Year	2010
Abstract	An effort has been made to investigate the chromatographic behavior and to understand the basic mechanisms in HILIC-based separation of water-soluble vitamins with highly varied properties on a diol column. The water content of the mobile phase is of utmost importance because it directly affects the type and extent of interactions of the solutes with the stationary phase and with the buffered mobile phase. A mixed-mode partitioning-surface adsorption mechanism enables most precise description of their chromatographic retention and separation. The point at which surface adsorption becomes apparent, however, depends on the properties of the solutes on the given stationary phase, and on the presence of buffer salt ions. Adjustment of mobile phase pH and use of different buffer salts can be used to modify electrostatic interactions among the solutes, active silanols, and counter-ions. The role of hydrogen bonding was clarified by substitution of ACN by solvents with moderate to strong hydrogen bonding potential. Analytes which are neutral at the working pH start to interact with the stationary phase when the ACN content is increased to 80%. Negatively charged analytes are adsorbed on the stationary phase when the ACN content is approximately 86%, because augmentation of the counter-ions weakens electrostatic repulsion by the active silanol groups. On the other hand, the electrostatic attraction of thiamine contributes significantly to its retention even when using mobile phases with high water content.
Starting Page	751
Ending Page	759
Page Count	9
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	71
Issue Number	9-10
e-ISSN	16121112
Language	English
Publisher	Vieweg Verlag
Publisher Date	2010-04-11
Publisher Place	Wiesbaden
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Column liquid chromatography Hydrophilic interaction liquid chromatography Diol column Chromatographic behavior Retention models Water-soluble vitamins Measurement Science and Instrumentation Plant Sciences Biochemistry Pharmacy Organic Chemistry Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Chromatographic Behavior of Uric Acid and Methyl Uric Acids on a Diol Column in HILIC

Analysis of Pterins in Urine by HILIC

Retention behavior of multiple sulfonamides in various liquid chromatographic systems

Novel Polymer Monolithic Column for Hydrophilic Compounds

A Stability-Indicating Assay of Brimonidine Tartrate Ophthalmic Solution and Stress Testing Using HILIC

Simultaneous determination of twelve water- and fat-soluble vitamins by high-performance liquid chromatography with diode array detection

Determination of water-soluble vitamins in pharmaceutical preparations by reversed-phase high-performance liquid chromatography with a mobile phase containing sodium dodecylsulphate andn-propanol

Modelling of RP chromatographic systems

Retention and column efficiency in reversed phase liquid chromatography as a function of pH for optimization purposes

Study of the Behavior of Water-Soluble Vitamins in HILIC on a Diol Column

Similar Documents

Chromatographic Behavior of Uric Acid and Methyl Uric Acids on a Diol Column in HILIC

Analysis of Pterins in Urine by HILIC

Retention behavior of multiple sulfonamides in various liquid chromatographic systems

Novel Polymer Monolithic Column for Hydrophilic Compounds

A Stability-Indicating Assay of Brimonidine Tartrate Ophthalmic Solution and Stress Testing Using HILIC

Simultaneous determination of twelve water- and fat-soluble vitamins by high-performance liquid chromatography with diode array detection

Determination of water-soluble vitamins in pharmaceutical preparations by reversed-phase high-performance liquid chromatography with a mobile phase containing sodium dodecylsulphate andn-propanol

Modelling of RP chromatographic systems

Retention and column efficiency in reversed phase liquid chromatography as a function of pH for optimization purposes

Study of the Behavior of Water-Soluble Vitamins in HILIC on a Diol Column