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| Content Provider | Springer Nature Link |
|---|---|
| Author | Lin, Dan Zhang, Yuan Wang, Xuebao Zhang, Zhennan Zhu, Jiayin Ye, Faqing Ye, Yaozhen Wang, Xianqin |
| Copyright Year | 2010 |
| Abstract | A sensitive and selective LC–MS–MS method for the determination of aminopyrine in human plasma was developed and validated over the concentration range of 8–8,000 ng mL−1. After addition of carbamazepine as internal standard, a simplified protein precipitation with methanol was employed for the sample preparation. Chromatographic separation was performed on an Agilent Zorbax SB-C18 column with a mobile phase of methanol–water. The MS data acquisition was accomplished in multiple reactions monitoring mode with a positive electrospray ionization interface. The lower limit of quantification was 8 ng mL−1. For inter-day and intra-day tests, the precision (RSD) for the entire validation was less than 9%, and the accuracy was within the 97.8–106.3% range. The method is simple and fast, and suitable for application to the analysis of samples for clinical intoxication. |
| Starting Page | 927 |
| Ending Page | 931 |
| Page Count | 5 |
| File Format | |
| ISSN | 00095893 |
| Journal | Chromatographia |
| Volume Number | 71 |
| Issue Number | 9-10 |
| e-ISSN | 16121112 |
| Language | English |
| Publisher | Vieweg Verlag |
| Publisher Date | 2010-04-01 |
| Publisher Place | Wiesbaden |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Column liquid chromatography Tandem mass spectrometry Aminopyrine Human plasma Measurement Science and Instrumentation Plant Sciences Biochemistry Pharmacy Organic Chemistry Analytical Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry |
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