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| Content Provider | Springer Nature Link |
|---|---|
| Author | Guo, Xiao Feng Li, Yun Wang, Hong Zhang, Hua Shan |
| Copyright Year | 2014 |
| Abstract | 1,3,5,7-Tetramethyl-8-daminozide-difluoroboradiaza-s-indacence (TMBODIPY-H), a pre-column fluorescent derivatization reagent, was applied to the analysis of fatty acid by high-performance liquid chromatography with fluorescence detection. Using this reagent, 12 fatty acids from propionic acid (C3) to stearic acid (C18) can be derivatized at room temperature in the presence of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide within 2 h. The baseline separation of these derivatives can be achieved within 46 min by gradient elution. The detection limits of these fatty acids are in the range of 0.1–1 nM and the linear ranges of most of them are 0.5–100 nM. This was the first application of hydrazine-based difluoro-boraindacene reagent for the analysis of fatty acids, and the proposed method has been successfully used for the determination of saliva samples of smokers and nonsmokers with recoveries of 88–110 %. |
| Starting Page | 431 |
| Ending Page | 438 |
| Page Count | 8 |
| File Format | |
| ISSN | 00095893 |
| Journal | Chromatographia |
| Volume Number | 77 |
| Issue Number | 5-6 |
| e-ISSN | 16121112 |
| Language | English |
| Publisher | Springer Berlin Heidelberg |
| Publisher Date | 2014-02-05 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | High-performance liquid chromatography Fluorescence detection Fatty acid 1,3,5,7-Tetramethyl-8-daminozide-difluoroboradiaza-s-indacence Chromatography Proteomics Pharmacy Laboratory Medicine |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry |
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