NDLI: Lipid Raft Stationary Phase Chromatography for Screening Anti-tumor Components from Galla chinensis

Content Provider	Springer Nature Link
Author	Tong, Shanshan Fu, Min Cao, Xia Firempong, Caleb Kesse Yi, Chengxue Zhen, Qianfeng Zhong, Hui Yu, Jiangnan Xu, Ximing
Copyright Year	2014
Abstract	For the first time, a novel biological affinity separating system called lipid raft stationary phase chromatography (LRSC) was developed. It was employed to screen bioactive components from Chinese gallnut, a traditional Chinese medicine (TCM). The LRSC was prepared by the addition of activated silica to Tris–HCl solution, which contains the isolated lipid rafts of U251 cells. This was followed by agitation, washing, centrifugation and then re-suspension of the residue in another Tris–HCl solution. The lipid rafts possess abundant receptor tyrosine kinase, specifically tropomyosin-related kinase A (TrkA), which is a widely researched anti-tumor drug target. Thus, TrkA provided the LRSC model with the ability to select fractions that specifically interact with it. Using a non-TrkA targeted anti-tumor drug (gemcitabine) and TrkA targeted anti-tumor drugs (lestaurtinib and gefitinib) as controls to evaluate the specific affinity of the LRSC column, the different fractions of Chinese gallnut were subjected to LRSC screening for the identification of anti-tumor components. As a result, the ether fraction of Chinese gallnut manifested desirable affinity properties. The methyl thiazolyl tetrazolium assay confirmed the anti-tumor effect of the screened ether fraction, and more importantly, the ether fraction failed woefully to exhibit its anti-proliferative activity in the presence of TrkA inhibitors (K252a and primary antibody). This further proves the selectivity of LRSC on TrkA-targeted drugs. The LRSC model has, therefore, shown to be of high efficiency and selectivity in screening bioactive components from the complex TCM extracts, thus offering an effective approach for the development of anticancer natural products.
Starting Page	419
Ending Page	429
Page Count	11
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	77
Issue Number	5-6
e-ISSN	16121112
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-01-23
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Lipid raft stationary phase chromatography Anti-tumor experimental therapeutics Drug screening Chinese traditional medicine Chinese gallnut Chromatography Proteomics Pharmacy Laboratory Medicine
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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