NDLI: Determination of Atovaquone in Human Plasma by LC-MS-MS and Its Application to a Bioequivalence Study

Content Provider	Springer Nature Link
Author	Pingale, Satish Gangaram Nerurkar, Ketan K. Padgaonkar, Ajit M. Pawar, Uttam D. Mangaonkar, Kiran V.
Copyright Year	2009
Abstract	A sensitive and selective method for the determination of atovaquone in human plasma was developed and validated. The procedure employed the use of an internal standard (chlorothalidone) and a solvent extraction step. Detection was by electrospray ionization tandem mass spectrometry with multiple reaction monitoring. The method showed a linear range from 50 to 2,000 ng mL−1. The extraction recovery was determined to be 84.91 ± 6.42% (SD), the intra- and inter-day assay accuracy (relative error) was within 7.57% and precision (RSD) was below 6.06%. The method was successfully employed to analyze plasma samples and evaluate the pharmacokinetics of atovaquone in human volunteers.
Starting Page	947
Ending Page	951
Page Count	5
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	70
Issue Number	5-6
e-ISSN	16121112
Language	English
Publisher	Vieweg Verlag
Publisher Date	2009-07-10
Publisher Place	Wiesbaden
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Column liquid chromatography-mass spectrometry Pharmacokinetics Antimalarial Atovaquone Human plasma Measurement Science and Instrumentation Plant Sciences Biochemistry Pharmacy Organic Chemistry Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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Determination of Atovaquone in Human Plasma by LC-MS-MS and Its Application to a Bioequivalence Study