NDLI: LC–MS Determination of Sterols in Olive Oil

Content Provider	Springer Nature Link
Author	Martínez Vidal, J. L. Garrido Frenich, A. Escobar García, M. A. Romero González, R.
Copyright Year	2007
Abstract	Sterols in olive oils have been analyzed by liquid chromatography coupled to mass spectrometry with atmospheric-pressure chemical ionization in positive-ion mode. A simple procedure based on saponification and extraction of the compounds from olive oils was studied. Validation of the method included calibration and determination of recovery and repeatability was carried out. Good linearity was obtained up to 100 mg kg−1 for all the sterols studied except β-sitosterol, for which linearity was obtained up to 2,000 mg kg−1. Recovery ranged from 88 to 110%, detection limits from 0.9 to 3.1 mg kg−1, and precision was good. The method has been successfully used for analysis of sterols in different types of oil. The predominant sterol was β-sitosterol; other minor components, for example sitostanol and cholesterol, were also detected. Total sterol content depended on the type of oil, and ranged from 687 to 2,479 mg kg−1. Stigmasterol and the amount of erythrodiol plus uvaol can be used to distinguish between olive oil and seed oil.
Starting Page	695
Ending Page	699
Page Count	5
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	65
Issue Number	11-12
e-ISSN	16121112
Language	English
Publisher	Vieweg Verlag
Publisher Date	2007-04-11
Publisher Place	Wiesbaden
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Column liquid chromatography–mass spectrometry Saponification Sterols in olive oil Erythrodiol and uvaol Measurement Science, Instrumentation Plant Sciences Biochemistry Pharmacy Organic Chemistry Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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