NDLI: Metabonomic Study of Lung Cancer and the Effects of Radiotherapy on Lung Cancer Patients: Analysis of Highly Polar Metabolites by Ultraperformance HILIC Coupled with Q-TOF MS

Content Provider	Springer Nature Link
Author	Cai, Xiaoming Dong, Jun Zou, Lijuan Xue, Xingya Zhang, Xiuli Liang, Xinmiao
Copyright Year	2011
Abstract	In this study, plasma samples were collected from 28 health volunteers and 66 lung cancer patients. Ultraperformance HILIC/Q-TOF MS-based metabonomic techniques were employed to compare the highly polar metabolites (HPMs) in the plasma of patients with lung cancer and those in the plasma of healthy volunteers. High separation efficiency and good repeatability of the HILIC/Q-TOF MS method were observed. Partial least squares discriminant analysis (PLS-DA) of the data from both ESI+ and ESI− mode revealed good classifications between the health volunteer and the lung cancer patients. This result showed that the HPMs from lung cancer patients and healthy controls were significantly different. The correct classification rate of models in both ion modes reached 100%, indicating that the metabonomics method established in this study could predict the plasma samples with high accuracy. Besides, we found 19 ions that show a significant difference in levels between lung cancer patients and healthy controls. Finally, we conducted a primary study of the effect of radiotherapy on HPMs in lung cancer patients. Clear differences among the HPMs of patients in different radiotherapy periods and significant changes in levels of the 19 ions after radiotherapy were noted. This demonstrated that radiotherapy influences the metabolism system of patients.
Starting Page	391
Ending Page	398
Page Count	8
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	74
Issue Number	5-6
e-ISSN	16121112
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-06-22
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	UPLC/Q-TOF Hydrophilic interaction chromatography Metabonomics Lung cancer Highly polar metabolites Radiotherapy Organic Chemistry Pharmacy Plant Sciences Proteomics Biochemistry Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Urinary Metabonomics Study of Heart Failure Patients with HILIC and RPLC Separation Coupled to TOF–MS

Identification of Metabolites of Epimedin A in Rats Using UPLC/Q–TOF–MS

Analysis of Metabolites of Anthraquinones by Human Fecal Bacteria Using UPLC-Q-TOF-HRMS/MS

UPLC-Q-TOF/MS for Analysis of the Metabolites of Flavone Glycosides from Scutellaria baicalensis Georgi by Human Fecal Flora in Vitro

Determination of Metabolism of Neohesperidin by Human Intestinal Bacteria by UPLC-Q-TOF/MS

UPLC-TOF MS-Based Metabonomic Study on Coadministration of Huperzine A and Ligustrazine Phosphate for Treatment of Alzheimer’s Disease

Application of UPLC-Quadrupole-TOF-MS Coupled with Recycling Preparative HPLC in Isolation and Preparation of Coumarin Isomers with Similar Polarity from Peucedanum praeruptorum

Search for Potential Biomarkers by UPLC/Q-TOF–MS Analysis of Dynamic Changes of Glycerophospholipid Constituents of RAW264.7 Cells Treated With NSAID

HILIC-MS/MS Method for Analysis of Ephedrine in Internet-available Drugs

Metabonomic Study of Lung Cancer and the Effects of Radiotherapy on Lung Cancer Patients: Analysis of Highly Polar Metabolites by Ultraperformance HILIC Coupled with Q-TOF MS

Similar Documents

Urinary Metabonomics Study of Heart Failure Patients with HILIC and RPLC Separation Coupled to TOF–MS

Identification of Metabolites of Epimedin A in Rats Using UPLC/Q–TOF–MS

Analysis of Metabolites of Anthraquinones by Human Fecal Bacteria Using UPLC-Q-TOF-HRMS/MS

UPLC-Q-TOF/MS for Analysis of the Metabolites of Flavone Glycosides from Scutellaria baicalensis Georgi by Human Fecal Flora in Vitro

Determination of Metabolism of Neohesperidin by Human Intestinal Bacteria by UPLC-Q-TOF/MS

UPLC-TOF MS-Based Metabonomic Study on Coadministration of Huperzine A and Ligustrazine Phosphate for Treatment of Alzheimer’s Disease

Application of UPLC-Quadrupole-TOF-MS Coupled with Recycling Preparative HPLC in Isolation and Preparation of Coumarin Isomers with Similar Polarity from Peucedanum praeruptorum

Search for Potential Biomarkers by UPLC/Q-TOF–MS Analysis of Dynamic Changes of Glycerophospholipid Constituents of RAW264.7 Cells Treated With NSAID

HILIC-MS/MS Method for Analysis of Ephedrine in Internet-available Drugs

Metabonomic Study of Lung Cancer and the Effects of Radiotherapy on Lung Cancer Patients: Analysis of Highly Polar Metabolites by Ultraperformance HILIC Coupled with Q-TOF MS