NDLI: Identification of Metabolites of Epimedin A in Rats Using UPLC/Q–TOF

Content Provider	Springer Nature Link
Author	Cui, Li Xu, Fengjuan Jiang, Jun Sun, E. Zhang, Zhenhai Cheng, Xudong Wang, Jing Jia, Xiaobin
Copyright Year	2014
Abstract	Herba Epimedii (Epimedium) is a kind of tonic herb, widely used in China. Epimedin A is a major component of Herba Epimedii with bioactivities. Analysis of the metabolic profile in vivo plays a pivotal role in understanding how traditional Chinese medicine works. And the metabolites of epimedin A might influence the effects of Herba Epimedii. Moreover, the metabolic routes of epimedin A provide an important basis for safety evaluation. Until now, little has been known about the metabolism of epimedin A. The current study was designed to characterize the metabolic pathways of epimedin A in vivo. The metabolites in rat plasma, bile, feces, and urine were identified by UPLC/Q–TOF–MS analysis. A total of 27 metabolites from epimedin A were detected or tentatively identified. The major metabolic processes were hydrolysis, hydrogenation, hydroxylation, dehydrogenation, demethylation, and conjugation with glucuronic acid and different sugars. The present study revealed the metabolic pathways of epimedin A in rat for the first time, and epimedin A could undergo extensive phase I and phase II metabolism in rat. These findings would provide an important basis for the further study and clinical application of epimedin A. In addition, the results of this work have shown the feasibility of the UPLC/Q–TOF–MS approach for rapid and reliable characterization of metabolites.
Starting Page	1223
Ending Page	1234
Page Count	12
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	77
Issue Number	17-18
e-ISSN	16121112
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-07-11
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Epimedin A Metabolites UPLC/Q–TOF–MS Metabolic pathways Chromatography Proteomics Pharmacy Laboratory Medicine
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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