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| Content Provider | Springer Nature Link |
|---|---|
| Author | Li, Q. Jia, Y. Sun, L. X. Xu, L. Tong, L. Shen, Z. D. Liu, Y. L. Bi, K. S. |
| Copyright Year | 2006 |
| Abstract | For the first time a high-performance liquid chromatographic (HPLC) method, with liquid-liquid extraction and ultraviolet (UV) absorbance detection, has been developed for quantification of isofraxidin in rat plasma. The analysis was performed on a Diamonsil C18 column (200 mm × 4.6 mm i.d., 5 µm particle size) with acetonitrile–0.05% phosphoric acid, 26:74 (v/v), as isocratic mobile phase. The linear range was 0.05–8.0 µg mL−1 and the lower limit of quantification was 0.05 µg mL−1. The intra and inter-day relative standard deviation (RSD) for measurement of 0.25, 2.0, and 6.0 µg mL−1 quality-control (QC) samples ranged from 5.7 to 6.4% and from 6.3 to 7.9%, respectively. Accuracy, as relative error (RE), was from ±5.8% to ±7.3%. The method was validated for specificity, accuracy, and precision and was successfully used in a pharmacokinetic study of isofraxidin in rat plasma after administration of Ciwujia extract. |
| Starting Page | 249 |
| Ending Page | 253 |
| Page Count | 5 |
| File Format | |
| ISSN | 00095893 |
| Journal | Chromatographia |
| Volume Number | 63 |
| Issue Number | 5-6 |
| e-ISSN | 16121112 |
| Language | English |
| Publisher | Vieweg Verlag |
| Publisher Date | 2006-02-28 |
| Publisher Place | Wiesbaden |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Column liquid chromatography Pharmacokinetic study Isofraxidin Ciwujia extract Rat plasma Analytical Chemistry Organic Chemistry Biochemistry Plant Sciences Pharmacy Measurement Science, Instrumentation |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry |
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