NDLI: Optimal Template Removal from Molecularly Imprinted Polymers by Pressurized Hot Water Extraction

Content Provider	Springer Nature Link
Author	Batlokwa, Bareki Shima Mokgadi, Janes Nyokong, Tebello Torto, Nelson
Copyright Year	2011
Abstract	An optimal extraction method for the removal of templates from molecularly imprinted polymers (MIPs) is presented. The extraction method is based on pressurized hot water extraction (PHWE). PHWE was evaluated by application to three distinctly colored MIPs for chlorophyll (green), quercetin (yellow) and phthalocynine (dark blue) with subsequent monitoring of template removal and template bleeding by an ultraviolet spectrophotometer. The templates were washed-off and the extraction efficiency (EE) was compared to that of soxhlet and ultrasonic extraction methods. PHWE employed hot water at an optimal temperature of 220 °C, pressure of 50 bars and flow rate of 2 mL min−1 to thoroughly wash-off the respective templates from their MIPs. The EE evaluated for PHWE was over 99.6% for all the MIPs with no subsequent or minimal template bleeding (<0.01%). The washing procedure was simple and relatively fast as it was achieved in 70 min at the most. At 95% confidence level (n = 3), soxhlet and ultrasonic recorded EE that was not significantly different (<94.5% in all cases) from that of PHWE (>99.6% in all cases). Soxhlet and ultrasonic had washing procedures that were slower (over 18 h) and employed large quantities (400 mL) of organic solvents modified with acids. The percentage relative standard deviations (%RSD) for the EE and recovery results were less than 2.3% in all cases indicating the high reproducibility of the method. Overall, the three methods performed comparably in extracting templates. PHWE seems to be the method of choice as it employed water which poses no environmental threat.
Starting Page	589
Ending Page	593
Page Count	5
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	73
Issue Number	5-6
e-ISSN	16121112
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-02-02
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Pressurized hot water extraction Green method of extraction Molecularly imprinted polymers Template removal Pharmacy Plant Sciences Organic Chemistry Analytical Chemistry Biochemistry Proteomics
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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