NDLI: Further studies on the MEKC and MEEKC of UV-Absorbing compounds in human urine

Content Provider	Springer Nature Link
Author	Perreff, D. Melin, V. Jennings, M. Alfazema, L.
Copyright Year	2000
Abstract	Micellar electrokinetic capillary chromatography (MEKC), a mode of capillary electrophoresis, can separate cations, anions and uncharged molecules simultaneously and with high resolution. So it is ideal for separating endogenous and exogenous analytes in complex biofluids, such as urine. Our previous system used β-CD-enhanced SDS MEKC to aid, resolution and could resolve a large number of peaks from human urine in about 12 minutes.In this study we have investigated a number of alternative additives to enhance resolution even further. Various compounds have been claimed, in the literature, to improve MEKC e. g. mixed micelles, organic solvents, and urea. We investigated a number of such compounds as additives in our standard urine system but without much success. However some modified cyclodextrins were found to increase both resolution and the migration window.In particular sulphated β-cyclodextrin gave excellent improvements in both resolution and selectivity. The optimised conditions (6.2 mM S-β-CD in 25 mM sodium tetraborate, pH 10, 75 mM SDS) resolved 70 components in a normal urine pool in<15 minutes using<10 nl of urine. Faster separations but with some loss of resolution were obtained by injection from the detector end of the capillary (short-end injection).In addition we have investigated the use of microemulsion electrokinetic chromatography MEEKC but found that it offered no real advantages over MEKC for urine analysis.This optimised MEKC separation is simpler, faster and gives higher resolution than gradient RP-LC.
Starting Page	S14
Ending Page	S18
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	52
Issue Number	1
e-ISSN	16121112
Language	English
Publisher	Vieweg Verlag
Publisher Date	2000-01-01
Publisher Place	Wiesbaden
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Capillary electrophoresis Micellar electrokinetic capillary chromatography Short-end injection Microemulsion electrokinetic capillary chromatography Human urine Analytical Chemistry Organic Chemistry Pharmacy Biochemistry Plant Sciences Measurement Science, Instrumentation
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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