NDLI: Direct Analysis of Phenol, Catechol and Hydroquinone in Human Urine by Coupled-Column HPLC with Fluorimetric Detection

Content Provider	Springer Nature Link
Author	Marrubini, G. Calleri, E. Coccini, T. Castoldi, A. F. Manzo, L.
Copyright Year	2005
Abstract	Phenol, catechol, and hydroquinone, are urinary end-products of the metabolism of benzene, nutrients, drugs, and endogenous substances. Recent research demonstrated that phenol, catechol, and hydroquinone, may have themselves a role in the carcinogenicity of benzene and in mechanisms that lead to leukemia. In this respect there is the need of rapid, low-cost, and possibly direct methods to quantitate these phenolic metabolites. Three single-residue coupled-column HPLC methods with fluorimetric detection (LC-LC-FLD) are described for the direct quantitation of phenol, catechol, and hydroquinone, in human urine. After enzymatic hydrolysis of the corresponding beta-glucuronoconjugates and sulfates, urine was directly injected into the LC-LC analyzer. The LC-LC-FLD procedure allowed base-to-base separation of the target compounds from urine interferents and good linearity (r2 = 0.998) within the ranges studied (0.5–50 mg L−1 for phenol, 0.35–35 mg L−1 for catechol and 0.2–10 mg L−1 for hydroquinone). Despite the high background levels of these metabolites in human urine, within- and inter-session precision expressed as RSD% was better than 20% on spiked and on authentic urine samples obtained from benzene-exposed workers. Accuracy expressed as the recovery ratio between measured and nominal concentration in spiked urine was comprised between 93% and 115% for the three metabolites. The column switching system was fully automated and computer-controlled, and was applied to the determination of phenol, catechol, and hydroquinone in urine samples showing a sample throughput of at least 20–30 samples per day.
Starting Page	25
Ending Page	31
Page Count	7
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	62
Issue Number	1-2
e-ISSN	16121112
Language	English
Publisher	Vieweg Verlag
Publisher Date	2005-06-15
Publisher Place	Wiesbaden
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Column liquid chromatography Coupled-columns Phenols and hydroquinone Human urine analysis Analytical Chemistry Organic Chemistry Pharmacy Biochemistry Plant Sciences Measurement Science, Instrumentation
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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