NDLI: Supercritical fluid extraction of bile acids from bovine bile raw materials

Content Provider	Springer Nature Link
Author	Scalia, S. Williams, J. R. Shim, J. H. Law, B. Morgan, E. D.
Copyright Year	1998
Abstract	The use of supercritical fluid extraction (SFE) as an alternative to conventional liquid extraction has been investigated to conventional liquid extraction has been investigated for the isolation of bile acids (cholic acid, deoxycholic acid and chenodeoxycholic acid) from the solid material obtained by bovine bile hydrolysis. Initial studies indicated that bile acids are not soluble in supercritical CO2, consequently the addition of the polar modifier methanol to the CO2 extracting fluid was evaluated. Quantitative recoveries (>88.5%) were obtained for the bile acids in 20 min with 15% (v/v) methanol in CO2 at 220 atm and 70°C. A comparison of SFE with pyridine extraction was performed on samples of hydrolyzed crude bovine bile. The SFE method yielded recoveries higher than 82.7% compared with conventional liquid extraction. Moreover, SFE minimized sample handling, reduced the consumption of harmful solvents and provided conditions suitable for selective extractions of bile acids from bovine bile raw materials.
Starting Page	785
Ending Page	789
Page Count	5
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	48
Issue Number	11-12
e-ISSN	16121112
Language	English
Publisher	Vieweg Verlag
Publisher Date	1998-01-01
Publisher Place	Wiesbaden
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Gas chromatography-mass spectrometry Column liquid chromatography Supercritical fluid extraction Bile acids Bovine bile hydrolysates Analytical Chemistry Organic Chemistry Pharmacy Biochemistry Plant Sciences Measurement Science, Instrumentation
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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