NDLI: Elimination of formate production in Clostridium thermocellum

Content Provider	Springer Nature Link
Author	Rydzak, Thomas Lynd, Lee R. Guss, Adam M.
Copyright Year	2015
Abstract	The ability of Clostridium thermocellum to rapidly degrade cellulose and ferment resulting hydrolysis products into ethanol makes it a promising platform organism for cellulosic biofuel production via consolidated bioprocessing. Currently, however, ethanol yield is far below theoretical maximum due to branched product pathways that divert carbon and electrons towards formate, H2, lactate, acetate, and secreted amino acids. To redirect carbon and electron flux away from formate, genes encoding pyruvate:formate lyase (pflB) and PFL-activating enzyme (pflA) were deleted. Formate production in the resulting Δpfl strain was eliminated and acetate production decreased by 50 % on both complex and defined medium. The growth rate of the Δpfl strain decreased by 2.9-fold on defined medium and biphasic growth was observed on complex medium. Supplementation of defined medium with 2 mM formate restored Δpfl growth rate to 80 % of the parent strain. The role of pfl in metabolic engineering strategies and C1 metabolism is discussed.
Starting Page	1263
Ending Page	1272
Page Count	10
File Format	PDF
ISSN	13675435
Journal	Journal of Industrial Microbiology and Biotechnology
Volume Number	42
Issue Number	9
e-ISSN	14765535
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-07-11
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Cellulosic ethanol Clostridium thermocellum Pyruvate:formate lyase Metabolic engineering C1 metabolism Microbiology Biochemistry Inorganic Chemistry Genetic Engineering Biotechnology Bioinformatics
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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