NDLI: Exploring medium-chain-length polyhydroxyalkanoates production in the engineered yeast Yarrowia lipolytica

Content Provider	Springer Nature Link
Author	Gao, Cuijuan Qi, Qingsheng Madzak, Catherine Lin, Carol Sze Ki
Copyright Year	2015
Abstract	Medium-chain-length polyhydroxyalkanoates (mcl-PHAs) are a large class of biopolymers that have attracted extensive attention as renewable and biodegradable bio-plastics. They are naturally synthesized via fatty acid de novo biosynthesis pathway or β-oxidation pathway from Pseudomonads. The unconventional yeast Yarrowia lipolytica has excellent lipid/fatty acid catabolism and anabolism capacity depending of the mode of culture. Nevertheless, it cannot naturally synthesize PHA, as it does not express an intrinsic PHA synthase. Here, we constructed a genetically modified strain of Y. lipolytica by heterologously expressing PhaC1 gene from P. aeruginosa PAO1 with a PTS1 peroxisomal signal. When in single copy, the codon optimized PhaC1 allowed the synthesis of 0.205 % DCW of PHA after 72 h cultivation in YNBD medium containing 0.1 % oleic acid. By using a multi-copy integration strategy, PHA content increased to 2.84 % DCW when the concentration of oleic acid in YNBD was 1.0 %. Furthermore, when the recombinant yeast was grown in the medium containing triolein, PHA accumulated up to 5.0 % DCW with as high as 21.9 g/L DCW, which represented 1.11 g/L in the culture. Our results demonstrated the potential use of Y. lipolytica as a promising microbial cell factory for PHA production using food waste, which contains lipids and other essential nutrients.
Starting Page	1255
Ending Page	1262
Page Count	8
File Format	PDF
ISSN	13675435
Journal	Journal of Industrial Microbiology and Biotechnology
Volume Number	42
Issue Number	9
e-ISSN	14765535
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-07-08
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Medium-chain-length polyhydroxyalkanoates Yarrowia lipolytica PHA synthase Strain engineering Triolein Microbiology Biochemistry Inorganic Chemistry Genetic Engineering Biotechnology Bioinformatics
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Carbon-limited fed-batch production of medium-chain-length polyhydroxyalkanoates by a phaZ-knockout strain of Pseudomonas putida KT2440

Production and characterization of two medium-chain-length polydroxyalkanoates by engineered strains of Yarrowia lipolytica

Production of erythritol and mannitol by Yarrowia lipolytica yeast in media containing glycerol

Engineering Yarrowia lipolytica for poly-3-hydroxybutyrate production

Increasing medium chain fatty acids production in Yarrowia lipolytica by metabolic engineering

Yarrowia lipolytica: the novel and promising 2-phenylethanol producer

Enhanced production of erythritol by Yarrowia lipolytica on glycerol in repeated batch cultures

High-yield production of citric acid by Yarrowia lipolytica on glycerol in repeated-batch bioreactors

Production of copolyesters of 3-hydroxybutyrate and medium-chain-length 3-hydroxyalkanoates by E. coli containing an optimized PHA synthase gene

Exploring medium-chain-length polyhydroxyalkanoates production in the engineered yeast Yarrowia lipolytica

Similar Documents

Carbon-limited fed-batch production of medium-chain-length polyhydroxyalkanoates by a phaZ-knockout strain of Pseudomonas putida KT2440

Production and characterization of two medium-chain-length polydroxyalkanoates by engineered strains of Yarrowia lipolytica

Production of erythritol and mannitol by Yarrowia lipolytica yeast in media containing glycerol

Engineering Yarrowia lipolytica for poly-3-hydroxybutyrate production

Increasing medium chain fatty acids production in Yarrowia lipolytica by metabolic engineering

Yarrowia lipolytica: the novel and promising 2-phenylethanol producer

Enhanced production of erythritol by Yarrowia lipolytica on glycerol in repeated batch cultures

High-yield production of citric acid by Yarrowia lipolytica on glycerol in repeated-batch bioreactors

Production of copolyesters of 3-hydroxybutyrate and medium-chain-length 3-hydroxyalkanoates by E. coli containing an optimized PHA synthase gene

Exploring medium-chain-length polyhydroxyalkanoates production in the engineered yeast Yarrowia lipolytica