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| Content Provider | Springer Nature Link |
|---|---|
| Author | Shen, Yanbing Yu, Ziqi Yang, Xu Wang, Fang Luo, Jianmei Wang, Min |
| Copyright Year | 2016 |
| Abstract | Cyclodextrins (CDs) can improve the productivity of steroid biotransformation by enhancing substrate solubility. CDs can be recycled by grafting them with appropriate carriers. Loofah fiber is an excellent grafting material for CDs, and can be applied to the biotransformation and recycling of β-cyclodextrin (β-CD). In this work, a technique for recycling β-CD in cortisone acetate (CA) biotransformation by Arthrobacter simplex CPCC 140451 was studied. Loofah fiber-grafted β-CD (LF-β-CD) was prepared using epichlorohydrin, which is a cross-linking agent. The grafting yield of β-CD was 74.8 mg g−1 dried fibers. LF-β-CD could increase the solubility of CA and enhance biotransformation. The initial conversion rate of CA was 1.5-fold higher than that of the blank group. LF-β-CD was also used in biocatalytic reactions for eight cycles, and it maintained the conversion ratio of CA at approximately 90%. Given the above positive results, LF-β-CD can be utilized in biotechnological recycling applications. This method can also be applied to CD derivatives and hydrophobic compounds. |
| Starting Page | 1 |
| Ending Page | 7 |
| Page Count | 7 |
| File Format | |
| ISSN | 13675435 |
| Journal | Journal of Industrial Microbiology and Biotechnology |
| Volume Number | 44 |
| Issue Number | 1 |
| e-ISSN | 14765535 |
| Language | English |
| Publisher | Springer Berlin Heidelberg |
| Publisher Date | 2016-11-15 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | Subscribed |
| Subject Keyword | Loofah fiber β-cyclodcxtrin Graft Biotransformation Recycle Microbiology Biochemistry Inorganic Chemistry Genetic Engineering Biotechnology Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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