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| Content Provider | Springer Nature Link |
|---|---|
| Author | Lazar, Zbigniew Rossigl, Tristan Verbeke, Jonathan Crutz Le Coq, Anne Marie Nicaud, Jean Marc Robak, Małgorzata |
| Copyright Year | 2013 |
| Abstract | Yarrowia lipolytica requires the expression of a heterologous invertase to grow on a sucrose-based substrate. This work reports the construction of an optimized invertase expression cassette composed of Saccharomyces cerevisiae Suc2p secretion signal sequence followed by the SUC2 sequence and under the control of the strong Y. lipolytica pTEF promoter. This new construction allows a fast and optimal cleavage of sucrose into glucose and fructose and allows cells to reach the maximum growth rate. Contrary to pre-existing constructions, the expression of SUC2 is not sensitive to medium composition in this context. The strain JMY2593, expressing this new cassette with an optimized secretion signal sequence and a strong promoter, produces 4,519 U/l of extracellular invertase in bioreactor experiments compared to 597 U/l in a strain expressing the former invertase construction. The expression of this cassette strongly improved production of invertase and is suitable for simultaneously high production level of citric acid from sucrose-based media. |
| Starting Page | 1273 |
| Ending Page | 1283 |
| Page Count | 11 |
| File Format | |
| ISSN | 13675435 |
| Journal | Journal of Industrial Microbiology and Biotechnology |
| Volume Number | 40 |
| Issue Number | 11 |
| e-ISSN | 14765535 |
| Language | English |
| Publisher | Springer Berlin Heidelberg |
| Publisher Date | 2013-09-06 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | Subscribed |
| Subject Keyword | Invertase Yarrowia lipolytica Sucrose Citric acid Secretion Microbiology Biochemistry Inorganic Chemistry Genetic Engineering Biotechnology Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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