NDLI: The glutamine synthetase (GS2) genes in relation to grain protein content of durum wheat

Content Provider	Springer Nature Link
Author	Gadaleta, Agata Nigro, Domenica Giancaspro, Angelica Blanco, Antonio
Copyright Year	2011
Abstract	Glutamine synthetase (GS2) is a key enzyme in plant nitrogen metabolism responsible of the first step of ammonium assimilation and transformation into glutamine (an essential compound in the amino acid-biosynthetic pathway). The goal of the present study was to isolate and characterize GS2 genes and to assess the linkage with grain protein content (GPC), an important quantitative trait controlled by multiple genes. Here, we report the isolation of the complete glutamine synthetase gene sequences and their localization on the two homeologous chromosome 2A and 2B in durum wheat cvs. Ciccio and Svevo characterized by a different grain protein content. GS2-A2 located on 2A chromosome is comprised of 13 exons separated by 12 introns, and the allele sequence in the two cultivars were different for an insertion of 5 bp located in the third exon in the cv. Ciccio. The GS2-B2 has the same intron/exon structure, but the two cultivars differ for the insertion of a 33-bp sequence located in the second intron of the cv. Svevo. Specific primers were designed in the polymorphic region and amplified in a recombinant inbred line mapping population. The study localized GS genes (GS2-A2, GS2-B2 GSe, GSr) on chromosomes 2A, 2B, 4A, and 4B, where four significant QTLs for GPC were also located.
Starting Page	665
Ending Page	670
Page Count	6
File Format	PDF
ISSN	1438793X
Journal	Functional & Integrative Genomics
Volume Number	11
Issue Number	4
e-ISSN	14387948
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-07-14
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Glutamine synthetase (GS) Wheat QTL analysis Grain protein content Bioinformatics Plant Genetics & Genomics Cell Biology Animal Genetics and Genomics Biochemistry Microbial Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine

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