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| Content Provider | Springer Nature Link |
|---|---|
| Author | Lee, Jong Yeol Beom, Hye Rang Altenbach, Susan B. Lim, Sun Hyung Kim, Yeong Tae Kang, Chon Sik Yoon, Ung Han Gupta, Ravi Kim, Sun Tae Ahn, Sang Nag Kim, Young Mi |
| Copyright Year | 2016 |
| Abstract | Although it is well known that low-molecular-weight glutenin subunits (LMW-GS) from wheat affect bread and noodle processing quality, the function of specific LMW-GS proteins remains unclear. It is important to find the genes that correspond to individual LMW-GS proteins in order to understand the functions of specific proteins. The objective of this study was to link LMW-GS genes and haplotypes characterized using well known Glu-A3, Glu-B3, and Glu-D3 gene-specific primers to their protein products in a single wheat variety. A total of 36 LMW-GS genes and pseudogenes were amplified from the Korean cultivar Keumkang. These include 11 Glu-3 gene haplotypes, two from the Glu-A3 locus, two from the Glu-B3 locus, and seven from the Glu-D3 locus. To establish relationships between gene haplotypes and their protein products, a glutenin protein fraction was separated by two-dimensional gel electrophoresis (2-DGE) and 17 protein spots were analyzed by N-terminal amino acid sequencing and tandem mass spectrometry (MS/MS). LMW-GS proteins were identified that corresponded to all Glu-3 gene haplotypes except the pseudogenes. This is the first report of the comprehensive characterization of LMW-GS genes and their corresponding proteins in a single wheat cultivar. Our approach will be useful to understand the contributions of individual LMW-GS to the end-use quality of flour. |
| Starting Page | 269 |
| Ending Page | 279 |
| Page Count | 11 |
| File Format | |
| ISSN | 1438793X |
| Journal | Functional & Integrative Genomics |
| Volume Number | 16 |
| Issue Number | 3 |
| e-ISSN | 14387948 |
| Language | English |
| Publisher | Springer Berlin Heidelberg |
| Publisher Date | 2016-02-16 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Triticum aestivum L. LMW-GS genes Proteomics Wheat quality improvement Cell Biology Plant Genetics & Genomics Microbial Genetics and Genomics Biochemistry Bioinformatics Animal Genetics and Genomics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Medicine |
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