NDLI: Cold-Adapted Digestive Aspartic Protease of the Clawed Lobsters Homarus americanus and Homarus gammarus: Biochemical Characterization

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Cold-Adapted Digestive Aspartic Protease of the Clawed Lobsters Homarus americanus and Homarus gammarus: Biochemical Characterization

Content Provider	Springer Nature Link
Author	Rojo, Liliana García Carreño, Fernando Angeles Navarrete del Toro, Maria
Copyright Year	2012
Abstract	Aspartic proteinases in the gastric fluid of clawed lobsters Homarus americanus and Homarus gammarus were isolated to homogeneity by single-step pepstatin-A affinity chromatography; such enzymes have been previously identified as cathepsin D-like enzymes based on their deduced amino acid sequence. Here, we describe their biochemical characteristics; the properties of the lobster enzymes were compared with those of its homolog, bovine cathepsin D, and found to be unique in a number of ways. The lobster enzymes demonstrated hydrolytic activity against synthetic and natural substrates at a wider range of pH; they were more temperature-sensitive, showed no changes in the K M value at 4°C, 10°C, and 25°C, and had 20-fold higher k cat /K M values than bovine enzyme. The bovine enzyme was temperature-dependent. We propose that both properties arose from an increase in molecular flexibility required to compensate for the reduction of reaction rates at low habitat temperatures. This is supported by the fast denaturation rates induced by temperature.
Starting Page	87
Ending Page	96
Page Count	10
File Format	PDF
ISSN	14362228
Journal	Marine Biotechnology
Volume Number	15
Issue Number	1
e-ISSN	14362236
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-05-31
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Lobster Aspartic proteinase Cathepsin D Enzyme kinetics Freshwater & Marine Ecology Microbiology Zoology Engineering
Content Type	Text
Resource Type	Article
Subject	Aquatic Science Biotechnology

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Aspartic Cathepsin D Endopeptidase Contributes to Extracellular Digestion in Clawed Lobsters Homarus americanus and Homarus gammarus

Cold-Adapted Digestive Aspartic Protease of the Clawed Lobsters Homarus americanus and Homarus gammarus: Biochemical Characterization

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Cold-Adapted Digestive Aspartic Protease of the Clawed Lobsters Homarus americanus and Homarus gammarus: Biochemical Characterization

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