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| Content Provider | Springer Nature Link |
|---|---|
| Author | Lu, Xia Wang, Hongxia Liu, Baozhong Xiang, Jianhai |
| Copyright Year | 2012 |
| Abstract | The clam Meretrix meretrix is a member of widely cultured, commercially important clams. A marker–trait association analysis was performed using expressed sequence tag (EST) simple sequence repeat (SSR) markers for marker-assisted selection in M. meretrix. Three markers, MM1272, MM2034, and MM7721, were found to be significantly associated with quantitative trait loci (QTLs) controlling shell length (P < 0.0001) in clams of a fast-growing population (JSF) and a control population (JSC). The 144-bp allele of MM1272, the 154-bp allele of MM2034, and the 152- and 165-bp alleles of MM7721 showed a significantly higher frequency in the JSF population (17.65, 36.41, 28.67, and 29.33 %) than in the JSC population (4.65, 8.33, 3.47, and 5.56 %). The three markers showed lower values for the number of alleles and observed heterozygosity as well as a higher proportion of homozygotes in JSF than in JSC population. The three markers have been further confirmed in the high and low tails of another population (09G3SPSB); similarly, lower values for the number of alleles and observed heterozygosity as well as a higher proportion of homozygotes were found in 09G3SPSBH. The putative functions of the three gene fragments containing MM1272, MM2034, and MM7721 also suggested that the three SSR-containing genes might be involved in growth of M. meretrix. All the results suggest that the three EST-SSR markers associated with growth QTLs would be useful for marker-assisted selection in M. meretrix breeding. |
| Starting Page | 16 |
| Ending Page | 25 |
| Page Count | 10 |
| File Format | |
| ISSN | 14362228 |
| Journal | Marine Biotechnology |
| Volume Number | 15 |
| Issue Number | 1 |
| e-ISSN | 14362236 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2012-04-27 |
| Publisher Place | New York |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Meretrix meretrix EST-SSR QTL Selective genotyping Marker–trait association Freshwater & Marine Ecology Microbiology Zoology Engineering |
| Content Type | Text |
| Resource Type | Article |
| Subject | Aquatic Science Biotechnology |
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