NDLI: D-Xylose Isomerase from a Marine Bacterium, Vibrio sp. Strain XY-214, and d-Xylulose Production from β-1,3-Xylan

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D-Xylose Isomerase from a Marine Bacterium, Vibrio sp. Strain XY-214, and d-Xylulose Production from β-1,3-Xylan

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D-Xylose Isomerase from a Marine Bacterium, Vibrio sp. Strain XY-214, and d-Xylulose Production from β-1,3-Xylan

Content Provider	Springer Nature Link
Author	Umemoto, Yoshiaki Shibata, Toshiyuki Araki, Toshiyoshi
Copyright Year	2011
Abstract	The xylA gene from a marine bacterium, Vibrio sp. strain XY-214, encoding d-xylose isomerase (XylA) was cloned and expressed in Escherichia coli. The xylA gene consisted of 1,320-bp nucleotides encoding a protein of 439 amino acids with a predicted molecular weight of 49,264. XylA was classified into group II xylose isomerases. The native XylA was estimated to be a homotetramer with a molecular mass of 190 kDa. The purified recombinant XylA exhibited maximal activity at 60°C and pH 7.5. Its apparent K m values for d-xylose and d-glucose were 7.93 and 187 mM, respectively. Furthermore, we carried out d-xylulose production from β-1,3-xylan, a major cell wall polysaccharide component of the killer alga Caulerpa taxifolia. The synergistic action of β-1,3-xylanase (TxyA) and β-1,3-xylosidase (XloA) from Vibrio sp. strain XY-214 enabled efficient saccharification of β-1,3-xylan to d-xylose. d-Xylose was then converted to d-xylulose by using XylA from the strain XY-214. The conversion rate of d-xylose to d-xylulose by XylA was found to be approximately 40% in the presence of 4 mM sodium tetraborate after 2 h of incubation. These results demonstrated that TxyA, XloA, and XylA from Vibrio sp. strain XY-214 are useful tools for d-xylulose production from β-1,3-xylan. Because d-xylulose can be used as a source for ethanol fermentation by yeast Saccharomyces cerevisiae, the present study will provide a basis for ethanol production from β-1,3-xylan.
Starting Page	10
Ending Page	20
Page Count	11
File Format	PDF
ISSN	14362228
Journal	Marine Biotechnology
Volume Number	14
Issue Number	1
e-ISSN	14362236
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-04-26
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Caulerpa taxifolia d-Xylose isomerase β-1,3-Xylanase β-1,3-Xylosidase Vibrio sp. strain XY-214 β-1,3-Xylan Zoology Microbiology Engineering Freshwater & Marine Ecology
Content Type	Text
Resource Type	Article
Subject	Aquatic Science Biotechnology

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Purification and Characterization of β-1,3-Xylanase from a Marine Bacterium, Vibrio sp. XY-214

Cloning, Sequencing, and Expression in Escherichia coli of the New Gene Encoding β-1,3-Xylanase from a Marine Bacterium, Vibrio sp. Strain XY-214

Purification and characterization of β-1,3-xylanase from a marine bacterium, Alcaligenes sp. XY-234

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D-Xylose Isomerase from a Marine Bacterium, Vibrio sp. Strain XY-214, and d-Xylulose Production from β-1,3-Xylan

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