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| Content Provider | Springer Nature Link |
|---|---|
| Author | Chung, Chih Ching Hwang, Sheng Ping L. Chang, Jeng |
| Copyright Year | 2008 |
| Abstract | In order to understand the molecular mechanism of growth regulation in phytoplankton and to develop novel growth-status indicators, a subtraction cDNA library was constructed by using the mRNA extracted from Skeletonema costatum in the rapid-growth stage (RG stage), and three RG-stage-related cDNA fragments, RG#14, RG#25, and RG#42, were obtained. According to the results of sequence analysis, RG#42 belonged to the MCM2-7 protein family, and the other two fragments, RG#14 and RG#25, were novel molecules. Under continuous illumination, these RG-stage-related mRNA expression levels increased from 100- (RG#14 and RG#42) to 1,000-fold (RG#25) with increasing growth rate. Furthermore, under a diel rhythm of light (light–dark = 12:12 h), the daily mean mRNA abundances of RG#14 and RG#25 in the exponential phase also differed from those in the late-stationary phase. However, such differences between these growth phases were not observed in the mRNA levels of RG#42 and PCNA. This study not only provided a new way to investigate the regulatory mechanisms of cell growth but also offered a possibility of employing these gene fragments as indicators to monitor the growth status of phytoplankton in the marine environment. |
| Starting Page | 356 |
| Ending Page | 367 |
| Page Count | 12 |
| File Format | |
| ISSN | 14362228 |
| Journal | Marine Biotechnology |
| Volume Number | 11 |
| Issue Number | 3 |
| e-ISSN | 14362236 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2008-10-08 |
| Publisher Place | New York |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Subtraction PCR Indicator Growth Diatom Engineering Zoology Microbiology Freshwater & Marine Ecology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Aquatic Science Biotechnology |
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