NDLI: Characterization and its Potential Application of Two Esterases Derived from the Arctic Sediment Metagenome

Content Provider	Springer Nature Link
Author	Jeon, Jeong Ho Kim, Jun Tae Kang, Sung Gyun Lee, Jung Hyun Kim, Sang Jin
Copyright Year	2008
Abstract	Two esterase genes (designated as estAT1 and estAT11, respectively) were cloned by activity-based screening of a fosmid library constructed with seashore sediment sample of the Arctic. The sequence analysis of the genes revealed that these esterase genes encoded proteins of 303 and 312 amino acids, respectively, and showed 40–50% identities to members of the hormone-sensitive lipase (HSL) family retaining a catalytic triad with a conserved GDSAG sequence and an oxyanion hole (HGGG). The esterases genes were overexpressed in Escherichia coli by co-expressing GroEL-GroES chaperonine, and the recombinant proteins (rEstAT1 and rEstAT11) were purified to homogeneity. The purified EstAT1 and EstAT11 were active in a broad range of temperature from 20 to 40°C with an optimum temperature at 30°C. The activation energies of rEstAT1 and rEstAT11 to hydrolyze p-nitrophenyl esters of butyrate were determined to be 12.65 kcal/mol and 11.26 kcal/mol, respectively, indicating that they are cold-adapted esterases. The purified EstAT1 and EstAT11 could hydrolyze racemic ofloxacin esters, and further rEstAT11 hydrolyzed preferentially (S)-racemic ofloxacin butyl ester with an enantiomeric excess (eep) value of 70.3%. This work represents an example that develops enzymes from the Arctic using metagenomic approach, potentially applicable to chiral resolution of heat-labile substrates.
Starting Page	307
Ending Page	316
Page Count	10
File Format	PDF
ISSN	14362228
Journal	Marine Biotechnology
Volume Number	11
Issue Number	3
e-ISSN	14362236
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-09-24
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Metagenome Arctic sediment Esterase Enantioselectivity Engineering Zoology Microbiology Freshwater & Marine Ecology
Content Type	Text
Resource Type	Article
Subject	Aquatic Science Biotechnology

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