NDLI: Amperometric tyrosinase biosensor based on boron-doped nanocrystalline diamond film electrode for the detection of phenolic compounds

Content Provider	Springer Nature Link
Author	Zou, Yousheng Lou, Dong Dou, Kang He, Linlin Dong, Yuhang Wang, Shalong
Copyright Year	2015
Abstract	A novel tyrosinase amperometric biosensor based on boron-doped nanocrystalline diamond (BDND) film electrode has been developed for the detection of phenolic compounds. Amine-terminated BDND surface was achieved via a direct photochemical reaction with allylamine. The tyrosinase was then covalently immobilized on the amine-terminated diamond film electrode surface. The effects of pH and applied potential on the performance of tyrosinase amperometric biosensor were investigated. The biosensor showed an optimum response at a pH of 6.5 and at an applied potential of −100 mV. It also exhibited good analytical performances to phenolic compounds in terms of sensitivity, detection limit, and stability. The tyrosinase-modified BDND film electrode exhibited a rapid response to the changes in the substrate concentration for all the phenolic compounds tested and reached 95 % of steady-state current in about 10 s. It is attributed to the high loading of tyrosinase and the rapid electron transfer between the tyrosinase and BDND film electrode surface. The tyrosinase-modified BDND film electrode showed high sensitivity with 184.0, 95.6, and 552.3 mA M−1 cm−2 and a linear response range of 10.0–120.0, 5.0–120.0, and 30.0–120.0 μM for phenol, catechol, and 4-chlorophenol, respectively.
Starting Page	47
Ending Page	54
Page Count	8
File Format	PDF
ISSN	14328488
Journal	Journal of Solid State Electrochemistry
Volume Number	20
Issue Number	1
e-ISSN	14330768
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-08-09
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Boron-doped nanocrystalline diamond film Tyrosinase Amperometric biosensor Phenolic compounds Physical Chemistry Electrochemistry Energy Storage Characterization and Evaluation of Materials Analytical Chemistry Condensed Matter Physics
Content Type	Text
Resource Type	Article
Subject	Condensed Matter Physics Materials Science Electrical and Electronic Engineering Electrochemistry

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