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Aluminum-tolerant Pseudomonas fluorescens: ROS toxicity and enhanced NADPH production

Content Provider	Springer Nature Link
Author	Singh, Ranji Beriault, Robin Middaugh, Jeffrey Hamel, Robert Chenier, Daniel Appanna, Vasu D. Kalyuzhnyi, Sergey
Copyright Year	2005
Abstract	Aluminum (Al) triggered a marked increase in reactive oxygen species (ROS) such as O2− and H2O2 in Pseudomonas fluorescens. Although the Al-stressed cells were characterized with higher amounts of oxidized lipids and proteins than controls, NADPH production was markedly increased in these cells. Blue native polyacrylamide gel electrophoresis (BN-PAGE) analyses coupled with activity and Coomassie staining revealed that NADP+ -dependent isocitrate dehydrogenase (ICDH, E.C. 1.1.1.42) and glucose-6-phosphate dehydrogenase (G6PDH, E.C. 1.1.1.49) played a pivotal role in diminishing the oxidative environment promoted by Al. These enzymes were overexpressed in the Al-tolerant microbes and were modulated by the presence of either Al or hydrogen peroxide (H2O2) or menadione. The activity of superoxide dismutase (SOD, E.C. 1.15.1.1), an enzyme known to combat ROS stress was also increased in the cells cultured in millimolar amounts of Al. Hence, Al-tolerant P. fluorescens invokes an anti-oxidative defense strategy in order to survive.
Starting Page	367
Ending Page	373
Page Count	7
File Format	PDF
ISSN	14310651
Journal	Extremophiles
Volume Number	9
Issue Number	5
e-ISSN	14334909
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-06-22
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Al toxicity ROS Isocitrate dehydrogenase Glucose 6 phosphate dehydrogenase Superoxide dismutase NADPH
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Medicine Microbiology

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