NDLI: Thermococcus kodakarensis has two functional PCNA homologs but only one is required for viability

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Thermococcus kodakarensis has two functional PCNA homologs but only one is required for viability

Content Provider	Springer Nature Link
Author	Pan, Miao Santangelo, Thomas J. Čuboňová, Ľubomíra Li, Zhuo Metangmo, Harlette Ladner, Jane Hurwitz, Jerard Reeve, John N. Kelman, Zvi
Copyright Year	2013
Abstract	Proliferating cell nuclear antigen (PCNA) monomers assemble to form a ring-shaped clamp complex that encircles duplex DNA. PCNA binding to other proteins tethers them to the DNA providing contacts and interactions for many other enzymes essential for DNA metabolic processes. Most eukarya and euryarchaea have only one PCNA homolog but Thermococcus kodakarensis uniquely has two, designated PCNA1 and PCNA2, encoded by TK0535 and TK0582, respectively. Here, we establish that both PCNA1 and PCNA2 form homotrimers that stimulate DNA synthesis by archaeal DNA polymerases B and D and ATP hydrolysis by the replication factor C complex. In exponentially growing cells, PCNA1 is abundant and present at an ~100-fold higher concentration than PCNA2 monomers. Deletion of TK0582 (PCNA2) had no detectable effects on viability or growth whereas repeated attempts to construct a T. kodakarensis strain with TK0535 (PCNA1) deleted were unsuccessful. The implications of these observations for PCNA1 function and the origin of the two PCNA-encoding genes in T. kodakarensis are discussed.
Starting Page	453
Ending Page	461
Page Count	9
File Format	PDF
ISSN	14310651
Journal	Extremophiles
Volume Number	17
Issue Number	3
e-ISSN	14334909
Language	English
Publisher	Springer Japan
Publisher Date	2013-03-24
Publisher Place	Japan
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Archaea DNA replication Genetics PCNA Structure Thermococcus kodakarensis Microbiology Biotechnology Biochemistry Microbial Ecology
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Medicine Microbiology

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Thermococcus kodakarensis has two functional PCNA homologs but only one is required for viability

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Thermococcus kodakarensis has two functional PCNA homologs but only one is required for viability

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Thermococcus kodakarensis has two functional PCNA homologs but only one is required for viability

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Thermococcus kodakarensis has two functional PCNA homologs but only one is required for viability