NDLI: Nitrosylation of rabbit ferrous heme-hemopexin

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Nitrosylation of rabbit ferrous heme-hemopexin

Content Provider	Springer Nature Link
Author	Fasa, Mauro Bocedi, Alessio Mattu, Marco Coletta, Massimo Ascenzi, Paolo
Copyright Year	2004
Abstract	Hemopexin (HPX) serves as a trap for toxic plasma heme, ensuring its complete clearance by transportation to the liver. Moreover, HPX-heme has been postulated to play a key role in the homeostasis of nitric oxide (NO). Here, the thermodynamics for NO binding to rabbit ferrous HPX-heme as well as the EPR and optical absorption spectroscopic properties of rabbit ferrous nitrosylated HPX-heme (HPX-heme-NO) are reported. The value of the dissociation equilibrium constant for NO binding to rabbit ferrous HPX-heme (i.e., H) is (1.4±0.2)×10−7 M, at pH 7.0 and 10.0 °C; the value of H is unaffected by sodium chloride. At pH 7.0, rabbit ferrous HPX-heme-NO is a six-coordinate heme-iron species, characterized by an X-band EPR spectrum with an axial geometry and by ε=146 mM−1 cm−1 at 419 nm. At pH 4.0, rabbit ferrous HPX-heme-NO is a five-coordinate heme-iron species, characterized by an X-band EPR spectrum with three-line splitting centered at 334 mT and by ε=74 mM−1 cm−1 at 387 nm. The pKa value of the reversible pH-induced six- to five-coordinate spectroscopic transition is 4.8±0.1 in the absence of sodium chloride and 4.3±0.1 in the presence of 1.5×10−1 M sodium chloride. This result is in agreement with the effect of sodium chloride on rabbit HPX-heme stability. The present data have been analyzed in parallel with those of a related heme model compound and heme-protein systems.
Starting Page	800
Ending Page	806
Page Count	7
File Format	PDF
ISSN	09498257
Journal	JBIC Journal of Biological Inorganic Chemistry
Volume Number	9
Issue Number	7
e-ISSN	14321327
Language	English
Publisher	Springer-Verlag
Publisher Date	2004-09-18
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Ferrous nitrosylated heme-hemopexin Heme-iron geometry NO binding properties Rabbit hemopexin Spectroscopic properties
Content Type	Text
Resource Type	Article
Subject	Biochemistry Inorganic Chemistry

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