NDLI: The 40s Ω-loop plays a critical role in the stability and the alkaline conformational transition of cytochrome c

Please wait, while we are loading the content...

Quantification of isomeric equilibria formed by metal ion complexes of 8-[2-(phosphonomethoxy)ethyl]-8-azaadenine (8,8aPMEA) and 9-[2-(phosphonomethoxy)ethyl]-8-azaadenine (9,8aPMEA). Derivatives of the antiviral nucleotide analogue 9-[2-(phosphonomethoxy)ethyl]adenine (PMEA)

IR and Raman study on the interactions of the 5′-GMP and 5′-CMP phosphate groups with Mg(II), Ca(II), Sr(II), Ba(II), Cr(III), Co(II), Cu(II), Zn(II), Cd(II), Al(III) and Ga(III)

Studies on the degradation pathway of iron-sulfur centers during unfolding of a hyperstable ferredoxin: cluster dissociation, iron release and protein stability

The 40s Ω-loop plays a critical role in the stability and the alkaline conformational transition of cytochrome c

Direct monitoring of the electron pool effect of cytochrome c3 by highly sensitive EQCM measurements

Miniaturized heme proteins: crystal structure of Co(III)-mimochrome IV

Structural and biochemical implications of single amino acid substitutions in the nucleotide-dependent switch regions of the nitrogenase Fe protein from Azotobacter vinelandii

Structure and binding of Mg(II) ions and di-metal bridge complexes with biological phosphates and phosphoranes

JBIC Journal of Biological Inorganic Chemistry : Volume 9, Issue 7, October 2004

JBIC Journal of Biological Inorganic Chemistry : Volume 9, Issue 6, September 2004

JBIC Journal of Biological Inorganic Chemistry : Volume 9, Issue 5, July 2004

JBIC Journal of Biological Inorganic Chemistry : Volume 9, Issue 4, June 2004

JBIC Journal of Biological Inorganic Chemistry : Volume 9, Issue 3, April 2004

JBIC Journal of Biological Inorganic Chemistry : Volume 9, Issue 2, March 2004

JBIC Journal of Biological Inorganic Chemistry : Volume 9, Issue 1, January 2004

JBIC Journal of Biological Inorganic Chemistry : Volume 8

JBIC Journal of Biological Inorganic Chemistry : Volume 7

JBIC Journal of Biological Inorganic Chemistry : Volume 6

JBIC Journal of Biological Inorganic Chemistry : Volume 5

JBIC Journal of Biological Inorganic Chemistry : Volume 4

JBIC Journal of Biological Inorganic Chemistry : Volume 3

JBIC Journal of Biological Inorganic Chemistry : Volume 2

The 40s Ω-loop plays a critical role in the stability and the alkaline conformational transition of cytochrome c

Content Provider	Springer Nature Link
Author	Caroppi, Paola Sinibaldi, Federica Santoni, Elisa Howes, Barry D. Fiorucci, Laura Ferri, Tommaso Ascoli, Franca Smulevich, Giulietta Santucci, Roberto
Copyright Year	2004
Abstract	The structural and redox properties of a non-covalent complex reconstituted upon mixing two non-contiguous fragments of horse cytochrome c, the residues 1–38 heme-containing N-fragment with the residues 57–104 C-fragment, have been investigated. With respect to native cyt c, the complex lacks a segment of 18 residues, corresponding, in the native protein, to an omega (Ω)-loop region. The fragment complex shows compact structure, native-like α-helix content but a less rigid atomic packing and reduced stability with respect to the native protein. Structural heterogeneity is observed at pH 7.0, involving formation of an axially misligated low-spin species and consequent partial displacement of Met80 from the sixth coordination position of the heme-iron. Spectroscopic data suggest that a lysine (located in the Met80-containing loop, namely Lys72, Lys73, or Lys79) replaces the methionine residue. The residues 1–38/57–104 fragment complex shows an unusual biphasic alkaline titration characterized by a low (pKa1=6.72) and a high pKa-associated state transition (pKa2=8.56); this behavior differs from that of native cyt c, which shows a monophasic alkaline transition (pKa=8.9). The data indicate that the 40s Ω-loop plays an important role in the stability of cyt c and in ensuring a correct alkaline conformational transition of the protein.
Starting Page	997
Ending Page	1006
Page Count	10
File Format	PDF
ISSN	09498257
Journal	JBIC Journal of Biological Inorganic Chemistry
Volume Number	9
Issue Number	8
e-ISSN	14321327
Language	English
Publisher	Springer-Verlag
Publisher Date	2004-10-19
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cytochrome c Fragment complex Intermediate state Loop regions
Content Type	Text
Resource Type	Article
Subject	Biochemistry Inorganic Chemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

The response of Ω-loop D dynamics to truncation of trimethyllysine 72 of yeast iso-1-cytochrome c depends on the nature of loop deformation

Conformational stability and dynamics of cytochrome c affect its alkaline isomerization

Insights into the role of the histidines in the structure and stability of cytochrome c

Conformational change and human cytochrome c function: mutation of residue 41 modulates caspase activation and destabilizes Met-80 coordination

Structural and thermodynamic behavior of cytochrome c assembled with glutathione-covered gold nanoparticles

Evolutionary alkaline transition in human cytochrome c

Disruption of a hydrogen bond network in human versus spider monkey cytochrome c affects heme crevice stability

Role of substrate on the conformational stability of the heme active site of cytochrome P450cam: effect of temperature and low concentrations of denaturants

Conformational and thermodynamic characterization of the premolten globule state occurring during unfolding of the molten globule state of cytochrome c

The 40s Ω-loop plays a critical role in the stability and the alkaline conformational transition of cytochrome c

Similar Documents

The response of Ω-loop D dynamics to truncation of trimethyllysine 72 of yeast iso-1-cytochrome c depends on the nature of loop deformation

Conformational stability and dynamics of cytochrome c affect its alkaline isomerization

Insights into the role of the histidines in the structure and stability of cytochrome c

Conformational change and human cytochrome c function: mutation of residue 41 modulates caspase activation and destabilizes Met-80 coordination

Structural and thermodynamic behavior of cytochrome c assembled with glutathione-covered gold nanoparticles

Evolutionary alkaline transition in human cytochrome c

Disruption of a hydrogen bond network in human versus spider monkey cytochrome c affects heme crevice stability

Role of substrate on the conformational stability of the heme active site of cytochrome P450cam: effect of temperature and low concentrations of denaturants

Conformational and thermodynamic characterization of the premolten globule state occurring during unfolding of the molten globule state of cytochrome c

The 40s Ω-loop plays a critical role in the stability and the alkaline conformational transition of cytochrome c