NDLI: In vivo monitoring of angiogenesis in a mouse hindlimb ischemia model using fluorescent peptide-based probes

Content Provider	Springer Nature Link
Author	Park, Subin Lee, Jangwook Jo, Mi hee Na, Jin Hee Park, Sung Gurl Jang, Hyeon Ki Kang, Sun Woong Kim, Jong Ho Kim, Byung Soo Park, Jae Hyung Kwon, Ick Chan Ryu, Ju Hee Kim, Kwangmeyung
Copyright Year	2016
Abstract	Vascular endothelial growth factor receptor (VEGFR) and matrix metalloproteinase (MMP) are up-regulated in ischemic tissue and play pivotal roles in promoting angiogenesis. The purpose of the present study was to evaluate two fluorophore-conjugated peptide probes specific to VEGFR and MMP for dual-targeted in vivo monitoring of angiogenesis in a murine model of hindlimb ischemia. To this end, VEGFR-Probe and MMP-Probe were developed by conjugating distinct near-infrared fluorophores to VEGFR-binding and MMP substrate peptides, respectively. VEGFR-Probe exhibited specific binding to VEGFR on HUVECs, and self-quenched MMP-Probe produced strong fluorescence intensity in the presence of MMPs in vitro. Subsequently, VEGFR-Probe and MMP-Probe were successfully utilized for time course in vivo visualization of VEGFR or MMP, respectively. Simultaneous visualization provided information regarding the spatial distribution of these proteins, including areas of co-localization. This dual-targeted in vivo imaging approach will be useful for understanding the detailed mechanism of angiogenesis and for evaluating therapeutic angiogenesis.
Starting Page	1641
Ending Page	1654
Page Count	14
File Format	PDF
ISSN	09394451
Journal	Amino Acids
Volume Number	48
Issue Number	7
e-ISSN	14382199
Language	English
Publisher	Springer Vienna
Publisher Date	2016-04-20
Publisher Place	Vienna
Access Restriction	Subscribed
Subject Keyword	Vascular endothelial growth factor receptor Matrix metalloproteinase Molecular imaging Angiogenesis Fluorescence imaging Biochemistry Analytical Chemistry Biochemical Engineering Life Sciences Proteomics Neurobiology
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Biochemistry Clinical Biochemistry

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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