NDLI: Knockdown of ornithine decarboxylase antizyme 1 causes loss of uptake regulation leading to increased N 1, N 11-bis(ethyl)norspermine (BENSpm) accumulation and toxicity in NCI H157 lung cancer cells

Content Provider	Springer Nature Link
Author	Fraser, Alison V. Goodwin, Andrew C. Hacker Prietz, Amy Sugar, Elizabeth Woster, Patrick M. Casero, Robert A.
Copyright Year	2011
Abstract	Ornithine decarboxylase antizyme 1 (AZ1) is a major regulatory protein responsible for the regulation and degradation of ornithine decarboxylase (ODC). To better understand the role of AZ1 in polyamine metabolism and in modulating the response to anticancer polyamine analogues, a small interfering RNA strategy was used to create a series of stable clones in human H157 non-small cell lung cancer cells that expressed less than 5–10% of basal AZ1 levels. Antizyme 1 knockdown clones accumulated greater amounts of the polyamine analogue N 1,N 11-bis(ethyl)norspermine (BENSpm) and were more sensitive to analogue treatment. The possibility of a loss of polyamine uptake regulation in the knockdown clones was confirmed by polyamine uptake analysis. These results are consistent with the hypothesis that AZ1 knockdown leads to dysregulation of polyamine uptake, resulting in increased analogue accumulation and toxicity. Importantly, there appears to be little difference between AZ1 knockdown cells and cells with normal levels of AZ1 with respect to ODC regulation, suggesting that another regulatory protein, potentially AZ2, compensates for the loss of AZ1. The results of these studies are important for the understanding of both the regulation of polyamine homeostasis and in understanding the factors that regulate tumor cell sensitivity to the anti-tumor polyamine analogues.
Starting Page	529
Ending Page	538
Page Count	10
File Format	PDF
ISSN	09394451
Journal	Amino Acids
Volume Number	42
Issue Number	2-3
e-ISSN	14382199
Language	English
Publisher	Springer Vienna
Publisher Date	2011-08-04
Publisher Place	Vienna
Access Restriction	Subscribed
Subject Keyword	Antizyme Ornithine decarboxylase Polyamines Spermine BENSpm Neurobiology Life Sciences Biochemistry Proteomics Biochemical Engineering Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Biochemistry Clinical Biochemistry

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Knockdown of Ornithine Decarboxylase Antizyme 1 causes loss of uptake regulation leading to increased N 1, N 11-bis(ethyl)norspermine (BENSpm) accumulation and toxicity in NCI H157 lung cancer cells

A role for antizyme inhibitor in cell proliferation

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Knockdown of ornithine decarboxylase antizyme 1 causes loss of uptake regulation leading to increased N 1, N 11-bis(ethyl)norspermine (BENSpm) accumulation and toxicity in NCI H157 lung cancer cells

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