NDLI: Functional consequences of actin nitration: in vitro and in disease states

Content Provider	Springer Nature Link
Author	Aslan, Mutay
Copyright Year	2010
Abstract	To link the phenomena of inflammatory-induced increases in protein nitrotyrosine (NO2Tyr) derivatives to protein dysfunction and consequent pathological conditions, the evaluation of discrete NO2Tyr modifications on specific proteins must be undertaken. Mass spectrometric (MS) proteomics-based strategies allow for the identification of all individual proteins that are nitrated by separating tissue homogenates using 2D gel electrophoresis, detecting the nitrated proteins using an anti-NO2Tyr antibody, and then identifying the peptides generated during an in-gel proteolytic digest using matrix-assisted laser desorption ionization/time-of-flight (MALDI-TOF) MS. Actin, one of the most abundant proteins in eukaryotic cells, constitutes 5% or more of cell protein and serves with other cytoskeletal proteins as a critical target for nitration-induced functional impairment. Herein, examples of actin nitration detected under physiological conditions in various models of human disease or in clinically derived tissues are given and the impact that this post-translational protein modification can have on cell and organ function is discussed.
Starting Page	65
Ending Page	74
Page Count	10
File Format	PDF
ISSN	09394451
Journal	Amino Acids
Volume Number	42
Issue Number	1
e-ISSN	14382199
Language	English
Publisher	Springer Vienna
Publisher Date	2010-05-18
Publisher Place	Vienna
Access Restriction	Subscribed
Subject Keyword	Nitrotyrosine Actin Reactive nitrogen species Reactive oxygen species Protein structure and function Biochemical Engineering Biochemistry Analytical Chemistry Neurobiology Proteomics Life Sciences
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Biochemistry Clinical Biochemistry

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