NDLI: Meiosis, Balbiani body and early asymmetry of Thermobia oocyte

Content Provider	Springer Nature Link
Author	Tworzydlo, Waclaw Marek, Magdalena Kisiel, Elzbieta Bilinski, Szczepan M.
Copyright Year	2016
Abstract	The meiotic division guarantees maintenance of a genetic diversity; it consists of several stages, with prophase I being the longest and the most complex. We decided to follow the course of initial stages of meiotic division in ovaries of Thermobia domestica using modified techniques of squash preparations, semithin sections, and electron microscopy. We show that germaria contain numerous germline cells that can be classified into three categories: cystoblasts, meiotic oocytes, and growing previtellogenic oocytes. The cystoblasts are located most apically. The meiotic oocytes occupy the middle part of the germarium, and the previtellogenic oocytes can be found in the most basal part, near the vitellarium. Analyses of the semithin sections and squash preparations show that post leptotene meiotic chromosomes gather in one region of the nucleoplasm where they form the so-called bouquet. The telomeres of the bouquet chromosomes are attached to a relatively small area (segment) of the nuclear envelope. Next to this envelope segment, the nucleolar organizers are also located. We show that in concert to sequential changes inside the oocyte nuclei, rearrangement of organelles within the ooplasm (oocyte cytoplasm) takes place. This leads to the formation of the Balbiani body and consequent asymmetry of the ooplasm. These early nuclear and cytoplasmic asymmetries, however, are transient. During diplotene, the chromosome bouquet disappears, while the Balbiani body gradually disperses throughout the ooplasm. Finally, our observations indicate the presence of lampbrush chromosomes in the nuclei of previtellogenic oocytes. In the close vicinity to lampbrush chromosomes, characteristic spherical nuclear bodies are present.
Starting Page	649
Ending Page	655
Page Count	7
File Format	PDF
ISSN	0033183X
Journal	Protoplasma
Volume Number	254
Issue Number	2
e-ISSN	16156102
Language	English
Publisher	Springer Vienna
Publisher Date	2016-05-14
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Oocyte Meiosis Prophase I Bouquet stage Lampbrush chromosomes Nucleolar organizer Cell Biology Plant Sciences Zoology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Plant Science Medicine

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Meiosis, Balbiani body and early asymmetry of Thermobia oocyte

“Bouquet arrest”, monopolar chromosomes segregation, and correction of the abnormal spindle

Are lampbrush chromosomes unique to meiotic cells?

Exclusion of dysfunctional mitochondria from Balbiani body during early oogenesis of Thermobia

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Exclusion of dysfunctional mitochondria from Balbiani body during early oogenesis of Thermobia

Lampbrush chromosomes enable study of cohesin dynamics

Meiosis, Balbiani body and early asymmetry of Thermobia oocyte

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Meiosis, Balbiani body and early asymmetry of Thermobia oocyte