NDLI: A rapid and efficient method to study the function of crop plant transporters in Arabidopsis

Content Provider	Springer Nature Link
Author	Wang, Xiangfeng Zhong, Fudi Woo, Cheuk Hang Miao, Yansong Grusak, Michael A. Zhang, Xiaobo Tu, Jumin Wong, Yum Shing Jiang, Liwen
Copyright Year	2016
Abstract	Iron (Fe) is an essential micronutrient for humans. Fe deficiency disease is widespread and has led to extensive studies on the mechanisms of Fe uptake and storage, especially in staple food crops such as rice. However, studies of functionally related genes in rice and other crops are often time and space demanding. Here, we demonstrate that transgenic Arabidopsis suspension culture cells and Arabidopsis plants can be used as an efficient expression system for gain-of-function study of selected transporters, using Fe transporters as a proof-of-principle. The vacuolar membrane transporters OsVIT1 and OsVIT2 have been described to be important for iron sequestration, and disruption of these two genes leads to Fe accumulation in rice seeds. In this study, we have taken advantage of the fluorescent-tagged protein GFP-OsVIT1, which functionally complements the Fe hypersensitivity of ccc1 yeast mutant, to generate transgenic Arabidopsis suspension cell lines and plants. GFP-OsVIT1 was shown to localize on the vacuolar membrane using confocal microscopy and immunogold EM. More importantly, the Fe concentration, as well as the concentration of Zn, in the transgenic cell lines and plants were significantly increased compared to that in the WT. Taken together, our study shows that the heterologous expression of rice vacuolar membrane transporter OsVIT1 in Arabidopsis system is functional and effectively enhances iron accumulation, indicating an useful approach for studying other putative transporters of crop plants in this system.
Starting Page	737
Ending Page	747
Page Count	11
File Format	PDF
ISSN	0033183X
Journal	Protoplasma
Volume Number	254
Issue Number	2
e-ISSN	16156102
Language	English
Publisher	Springer Vienna
Publisher Date	2016-05-30
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	OsVIT1 Iron accumulation Arabidopsis suspension culture cells Cell Biology Plant Sciences Zoology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Plant Science Medicine

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