NDLI: Development of novel antibodies against non-structural proteins nsP1, nsP3 and nsP4 of chikungunya virus: potential use in basic research

Content Provider	Springer Nature Link
Author	Kumar, Sameer Mamidi, Prabhudutta Kumar, Abhishek Basantray, Itishree Bramha, Umarani Dixit, Anshuman Maiti, Prasanta Kumar Singh, Sujay Suryawanshi, Amol Ratnakar Chattopadhyay, Subhasis Chattopadhyay, Soma
Copyright Year	2015
Abstract	Chikungunya virus (CHIKV) has reemerged recently as an important pathogen, causing several large epidemics worldwide. This necessitates the development of better reagents to understand its biology and to establish effective and safe control measures. The present study describes the development and characterization of polyclonal antibodies (pAbs) against synthetic peptides of CHIKV non-structural proteins (nsPs; nsP1, nsP3 and nsP4). The reactivity of these pAbs was demonstrated by ELISA and Western blot. Additionally, in vitro infection studies in a mammalian system confirmed that these pAbs are highly sensitive and specific for CHIKV nsPs, as these proteins were detected very early during viral replication. Homology analysis of the selected epitope sequences revealed that they are conserved among all of the CHIKV strains of different genotypes, while comparison with other alphavirus sequences showed that none of them are 100 % identical to the epitope sequences (except Onyong-nyong and Igbo Ora viruses, which show 100 % identity to the nsP4 epitope). Interestingly, two different forms of CHIKV nsP1 and three different forms of nsP3 were detected in Western blot analysis during infection; however, further experimental investigations are required to confirm their identity. Also, the use of these antibodies demonstrated faster and enhanced expression profiles of all CHIKV nsPs in 2006 Indian outbreak strains when compared to the CHIKV prototype strain, suggesting the epidemic potential of the 2006 isolate. Accordingly, it can be suggested that the pAbs reported in this study can be used as sensitive and specific tools for experimental investigations of CHIKV replication and infection.
Starting Page	2749
Ending Page	2761
Page Count	13
File Format	PDF
ISSN	03048608
Journal	Archives of Virology
Volume Number	160
Issue Number	11
e-ISSN	14328798
Language	English
Publisher	Springer Vienna
Publisher Date	2015-08-18
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Virology Medical Microbiology Infectious Diseases
Content Type	Text
Resource Type	Article
Subject	Virology Medicine

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