NDLI: Electrochemical method for monitoring the progress of polymerase chain reactions using Methylene blue as an indicator

Content Provider	Springer Nature Link
Author	Wang, Kun Chen, Yi Ping Lei, Yun Zhong, Guang xian Liu, Ai lin Zheng, Yan Jie Sun, Zhou Liang Lin, Xin hua Chen, Yuan zhong
Copyright Year	2013
Abstract	We report on the proof-of-principle of an amperometric method to monitor the progress of a polymerase chain reaction (PCR) in real-time. It is based on the finding that the intercalating redox probe Methylene Blue (MB) becomes less easily electrochemically detectable once intercalated into double-stranded DNA (ds-DNA) compared to its free form. This was studied by cyclic voltammetry before and after addition of salmon sperm ds-DNA. Under optimized conditions, the products of the PCR of mitochondrial DNA fragments were quantitatively detected at different stages of amplification cycles. This strategy is potentially cheaper and easier to integrate into a hand-held miniaturized device than fluorescence-based real-time PCR. Figure We report on the proof-of-principle of an amperometric method based on methylene blue which becomes less electrochemically detectable once intercalated into double-stranded DNA compared to its free form to monitor the progress of PCR in real-time. This strategy is potentially cheap and easy to integrate into a hand-held miniaturized device.
Starting Page	871
Ending Page	878
Page Count	8
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	180
Issue Number	9-10
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2013-05-03
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Real-time polymerase chain reaction Electrochemical monitoring Methylene blue Deoxyribonucleic acid indicator Polymerase chain reaction products Nanochemistry Nanotechnology Characterization and Evaluation of Materials Analytical Chemistry Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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