NDLI: Label-free electrochemical immunosensor for the carcinoembryonic antigen using a glassy carbon electrode modified with electrodeposited Prussian Blue, a graphene and carbon nanotube assembly and an antibody immobilized on gold nanoparticles

Content Provider	Springer Nature Link
Author	Feng, Dexiang Lu, Xiaocui Dong, Xiao Ling, Yunyun Zhang, Yuzhong
Copyright Year	2013
Abstract	We described a sensitive, label-free electrochemical immunosensor for the detection of carcinoembryonic antigen. It is based on the use of a glassy carbon electrode (GCE) modified with a multi-layer films made from Prussian Blue (PB), graphene and carbon nanotubes by electrodeposition and assembling techniques. Gold nanoparticles were electrostatically absorbed on the surface of the film and used for the immobilization of antibody, while PB acts as signaling molecule. The stepwise assembly process was investigated by differential pulse voltammetry and scanning electron microscopy. It is found that the formation of antibody-antigen complexes partially inhibits the electron transfer of PB and decreased its peak current. Under the optimal conditions, the decrease of intensity of the peak current of PB is linearly related to the concentration of carcinoembryonic antigen in two ranges (0.2–1.0, and 1.0–40.0 ng·mL−1), with a detection limit of 60 pg·mL−1 (S/N = 3). The immunosensor was applied to analyze five clinical samples, and the results obtained were in agreement with clinical data. In addition, the immunosensor exhibited good precision, acceptable stability and reproducibility. Figure We described a sensitive electrochemical immunosensor for the detection of the carcinoembryonic antigen. It was based on the use of a glassy carbon electrode modified with a multi-layer films made from Prussian blue, graphene, and carbon nanotubes by electrodeposition and assembling techniques. The immunosensor exhibited good precision and acceptable stability and has been applied to analyze clinical sample with a satisfactory result.
Starting Page	767
Ending Page	774
Page Count	8
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	180
Issue Number	9-10
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2013-04-06
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Immunosensor Carcinoembryonic antigen Graphene Prussian blue Carbon nanotubes Gold nanoparticles Nanochemistry Nanotechnology Characterization and Evaluation of Materials Analytical Chemistry Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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