NDLI: Glassy carbon electrode modified with gold nanoparticles and hemoglobin in a chitosan matrix for improved pH-switchable sensing of hydrogen peroxide

Content Provider	Springer Nature Link
Author	Liu, Yang Shi, Liang Gong, Jin Fang, Yu Ting Bao, Ning Gu, Hai Ying Zeng, Jiang
Copyright Year	2015
Abstract	Hemoglobin (Hb) has been demonstrated to endow electrochemical sensors with pH-switchable response because of the presence of carboxyl and amino groups. Hb was deposited in a chitosan matrix on a glassy carbon electrode (GCE) that was previously coated with clustered gold nanoparticles (Au-NPs) by electrodeposition. The switching behavior is active (“on”) to the negatively charged probe [Fe(CN)6 3−] at pH 4.0, but inactive (“off”) to the probe at pH 8.0. This switch is fully reversible by simply changing the pH value of the solution and can be applied for pH-controlled reversible electrochemical reduction of H2O2 catalyzed by Hb. The modified electrode was tested for its response to the different electroactive probes. The response to these species strongly depends on pH which was cycled between 4 and 8. The effect is also attributed to the presence of pH dependent charges on the surface of the electrode which resulted in either electrostatic attraction or repulsion of the electroactive probes. The presence of Hb, in turn, enhances the pH-controllable response, and the electrodeposited Au-NPs improve the capability of switching. This study reveals the potential of protein based pH-switchable materials and also provides a simple and effective strategy for fabrication of switchable chemical sensors as exemplified in a pH-controllable electrode for hydrogen peroxide. Graphical Abstract A pH “on-off” switchable nanobiosensor was fabricated by casting a chitosan-hemoglobin biocomposite onto nano-gold electrode. This composite film exhibits not only excellent pH-responsive on (pH 4.0)-off (pH 8.0) behavior but also excellent pH-tunable on-off bioelectrocatalysis of H2O2.
Starting Page	2461
Ending Page	2468
Page Count	8
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	182
Issue Number	15-16
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2015-08-18
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Hemoglobin Gold nanoparticles Chitosan Electrochemistry Nanochemistry Nanotechnology Characterization and Evaluation of Materials Analytical Chemistry Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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