NDLI: A fluorine-doped tin oxide electrode modified with gold nanoparticles for electrochemiluminescent determination of hydrogen peroxide released by living cells

Content Provider	Springer Nature Link
Author	Li, Man Gao, Hongfang Wang, Xiaofei Wang, Yufeng Qi, Honglan Zhang, Chengxiao
Copyright Year	2016
Abstract	An electrogenerated chemiluminescence (ECL) based method is described for the determination of hydrogen peroxide (H2O2) released by living cells. It is making use of a fluorine-doped tin oxide (FTO) electrode that was modified with gold nanoparticles (AuNPs). The AuNP-FTO was fabricated by electrodeposition of AuNP on the surface of the FTO electrode and characterized by scanning electron microscopy and electrochemical techniques. The AuNP-FTO exhibits a significant electrocatalytic activity towards luminol-based ECL system compared to a bare FTO. The calibration plot covers the 10 nM to 1 μM H2O2 concentration range, with a 8 nM detection limit (at a signal-to-noise ratio of 3). The method enabled (a) the monitoring of drug-stimulated production of H2O2 released by living cells including RAW 264.7 macrophage cells and HeLa cells; and (b) the determination of inhibitors of H2O2 secretion. The method, due to strong signal amplification by AuNPs, provides a viable tool for determination of H2O2 in biological systems. Graphical abstract An electrogenerated chemiluminescence method was developed for determination of hydrogen peroxide using a fluorine-doped tin oxide electrode modified with gold nanoparticles. This method enabled the determination of drug-stimulated production of H2O2 released by living cells and inhibitor of H2O2 secretion.
Starting Page	603
Ending Page	610
Page Count	8
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	184
Issue Number	2
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2016-12-19
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	ECL FTO Gold nanoparticles Luminol Hydrogen peroxide Macrophage cells HeLa cells Nanochemistry Nanotechnology Characterization and Evaluation of Materials Analytical Chemistry Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Electrochemiluminescent detection of the hybridization of oligonucleotides using an electrode modified with nanocomposite of carbon nanotubes and gold nanoparticles

Voltammetric cadmium(II) sensor based on a fluorine doped tin oxide electrode modified with polyamide 6/chitosan electrospun nanofibers and gold nanoparticles

Self-assembly of ultra-small gold nanoparticles on an indium tin oxide electrode for the enzyme-free detection of hydrogen peroxide

New method for preparation of polyoxometalate-capped gold nanoparticles, and their assembly on an indium-doped tin oxide electrode

New synthesis of gold nanocorals using a diazonium compound, and their application to an electrochemiluminescent assay of hydrogen peroxide

Copper oxide nanoparticles and ionic liquid modified carbon electrode for the non-enzymatic electrochemical sensing of hydrogen peroxide

Electrochemical hydrogen peroxide sensor based on a glassy carbon electrode modified with nanosheets of copper-doped copper(II) oxide

Determination of hydrogen peroxide using a Prussian Blue modified macroporous gold electrode

Non-enzymatic hydrogen peroxide sensor using an electrode modified with iron pentacyanonitrosylferrate nanoparticles

A fluorine-doped tin oxide electrode modified with gold nanoparticles for electrochemiluminescent determination of hydrogen peroxide released by living cells

Similar Documents

Electrochemiluminescent detection of the hybridization of oligonucleotides using an electrode modified with nanocomposite of carbon nanotubes and gold nanoparticles

Voltammetric cadmium(II) sensor based on a fluorine doped tin oxide electrode modified with polyamide 6/chitosan electrospun nanofibers and gold nanoparticles

Self-assembly of ultra-small gold nanoparticles on an indium tin oxide electrode for the enzyme-free detection of hydrogen peroxide

New method for preparation of polyoxometalate-capped gold nanoparticles, and their assembly on an indium-doped tin oxide electrode

New synthesis of gold nanocorals using a diazonium compound, and their application to an electrochemiluminescent assay of hydrogen peroxide

Copper oxide nanoparticles and ionic liquid modified carbon electrode for the non-enzymatic electrochemical sensing of hydrogen peroxide

Electrochemical hydrogen peroxide sensor based on a glassy carbon electrode modified with nanosheets of copper-doped copper(II) oxide

Determination of hydrogen peroxide using a Prussian Blue modified macroporous gold electrode

Non-enzymatic hydrogen peroxide sensor using an electrode modified with iron pentacyanonitrosylferrate nanoparticles

A fluorine-doped tin oxide electrode modified with gold nanoparticles for electrochemiluminescent determination of hydrogen peroxide released by living cells