NDLI: Enhanced amperometric immunoassay for the prostate specific antigen using Pt-Cu hierarchical trigonal bipyramid nanoframes as a label

Content Provider	Springer Nature Link
Author	Jiao, Lei Mu, Zonggang Miao, Luyang Du, Wenwen Wei, Qin Li, He
Copyright Year	2016
Abstract	A dual enhancing strategy has been employed to develop a sandwich type of electrochemical immunoassay for the prostate specific antigen (PSA). The signal is enhanced by using Pt-Cu hierarchical trigonal bipyramid nanoframes (HTBNFs) and a composite consisting of Fe3O4 nanoparticles and reduced graphene oxide in polydopamine that serve to capture the primary antibody (Ab1). This nanocomposite shows better electrical conductivity than Fe3O4 and reduced graphene oxide (RGO), respectively, alone. The Pt-Cu HTBNFs were used to label the secondary antibody (Ab2) and act as tags for signal amplification by virtue of their outstanding electrochemical reduction activity towards H2O2. At a working potential of +0.1 V (vs. SCE), the interference by dissolved oxygen can be avoided. This immunoassay is highly sensitive, with a linear range that extends from 0.1 pg⋅mL−1 to 5 ng⋅mL−1 and an ultralow detection limit of 0.03 pg⋅mL−1. Graphical abstract Schematic of the dual amplification strategy in the immunosensor for the prostate specific antigen (PSA) that is based on the use of a first antibody (Ab1) conjugated to a Fe3O4-reduced graphene oxide nanocomposite (Fe3O4-RGO), and of Pt-Cu trigonal bipyramid nanoframes as a label for the second antibody (Ab2).
Starting Page	423
Ending Page	429
Page Count	7
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	184
Issue Number	2
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2016-11-28
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Prostate specific antigen Dual enhancing strategy Interference by dissolved oxygen Ultrasensitive detection Cyclic voltammetry Nanochemistry Nanotechnology Characterization and Evaluation of Materials Analytical Chemistry Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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