NDLI: Determination of thiol compounds by solid-phase extraction using multi-walled carbon nanotubes as adsorbent coupled with high-performance liquid chromatography-fluorescence detection

Content Provider	Springer Nature Link
Author	Huang, Ke Jing Han, Cong Hui Han, Chao Qun Li, Jing Wu, Zhi Wei Liu, Yan Ming
Copyright Year	2011
Abstract	We describe a method for solid-phase extraction of biogenic thiols using multi-walled carbon nanotubes as adsorbent, and their subsequent determination via HPLC and fluorescence detection. The fluorogenic reagent N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-yl)methyl) iodoacetamide was applied to derivatizate the thiols. The type of eluent and its volume, the sample pH, extraction time and sample volume were optimized. The calibration curves of the thiols are linear in the range from 0.5 to 200 nM (for glutathione), 0.02 to 5 nM (for cysteine), and 2 to 500 nM (for acetylcysteine), and the correlation coefficients range between 0.9955 and 0.9997. The respective limits of detection are 20 pM, 4 pM and 80 pM (at an SNR of 3), and the limits of quatification are 67 pM, 13 pM, and 267 pM (at an SNR of 10). Recoveries range from 85.0% to 113.1% for human urine and plasma samples spiked with the three thiols, and relative standard deviations are in the range from 2.1 to 7.4%. Figure Chromatograms for thiols derivatives obtained by MWCNTs-based SPE-HPLC (A) and direct HPLC analysis (B).
Starting Page	421
Ending Page	427
Page Count	7
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	174
Issue Number	3-4
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2011-06-30
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	High-performance liquid chromatography-fluorescence detection Solid-phase extraction Multi-walled carbon nanotubes Thiol compounds Nanotechnology Microengineering Analytical Chemistry Characterization and Evaluation of Materials Nanochemistry
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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