NDLI: Label-free and ultrasensitive electrochemiluminescence detection of microRNA based on long-range self-assembled DNA nanostructures

Content Provider	Springer Nature Link
Author	Liu, Ting Chen, Xian Hong, Cheng Yi Xu, Xiao Ping Yang, Huang Hao
Copyright Year	2013
Abstract	Electrochemiluminescence (ECL) integrates the advantages of electrochemical detection and chemiluminescent techniques. The method has received particular attention because it is highly sensitive and selective, has a wide linear range but low reagent costs. The use of nanomaterials with their unique physical and chemical properties has led to new kinds of biosensors that exhibit high sensitivity and stability. Compared to other nanomaterials, DNA nanostructures are more biocompatible, more hydrophilic, and thus less prone to nonspecific adsorption onto the electrode surface. We describe here a label-free and ultrasensitive ECL biosensor for detecting a cancer-associated microRNA at a femtomolar level. We have designed two auxiliary probes that cause the formation of a long-range self-assembly in the form of a μm-long 1-dimensional DNA concatamer. These can be used as carriers for signal amplification. The intercalation of the ECL probe Ru(phen)3 2+ into the grooves of the concatamers leads to a substantial increase in ECL intensity. This amplified sensor shows high selectivity for discriminating complementary target and other mismatched RNAs. The biosensor enables the quantification of the expression of microRNA-21 in MCF-7 cells. It also displays very low limits of detection and provides an alternative approach for the detection of RNA or DNA detection in diagnostics and gene analysis. Figure The long-range self-assembly DNA concatamers were used as carriers for signal amplification by the intercalation of numerous ECL probe (Ru(phen)3 2+) into the grooves of the DNA concatamers. Such signal amplification strategy lead to a substantial increase in ECL intensity and sensitivity.
Starting Page	731
Ending Page	736
Page Count	6
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	181
Issue Number	7-8
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2013-11-13
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Electrochemiluminescence Nanomaterials DNA concatamers MicroRNA Nanochemistry Nanotechnology Characterization and Evaluation of Materials Analytical Chemistry Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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