NDLI: Selection and characterization of DNA aptamers against the steroid testosterone

Content Provider	Springer Nature Link
Author	Skouridou, Vasso Jauset Rubio, Miriam Ballester, Pablo Bashammakh, Abdulaziz S. El Shahawi, Mohammad S. Alyoubi, Abdulrahman O. O’Sullivan, Ciara K.
Copyright Year	2017
Abstract	Anabolic androgenic steroids (AAS) are frequently abused in human and animal sports as performance-enhancing drugs, and consequently their use is controlled by international sports authorities. Testosterone is one of the most frequently used AAS, and therefore the accurate determination of its levels in biological fluids is very important. The authors describe the selection of testosterone-binding aptamers performed using a classic SELEX approach with the target immobilized on magnetic beads. Counter selections with structurally similar steroids were implemented at different stages. Pools from different selection rounds were sequenced with Next Generation Sequencing and ten aptamer candidates were selected for further characterization. Low nanomolar range dissociation constants were calculated by a bead-based PCR assay and verified by microscale thermophoresis. Future work will focus on the development of aptamer-based platforms for the sensitive detection of testosterone in biological samples and the validation of these assays for the rapid screening of suspicious samples. Graphical abstract The selection of testosterone-binding aptamers is described via classic SELEX using the target immobilized on magnetic beads combined with Next Generation Sequencing. The process let to the identification of several unique aptamer candidates which were characterized and their binding to testosterone was evaluated.
Starting Page	1631
Ending Page	1639
Page Count	9
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	184
Issue Number	6
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2017-03-11
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Anabolic androgenic steroids Doping Aptamer SELEX Next-generation sequencing apta-PCR affinity assay Microscale thermophoresis Nanochemistry Nanotechnology Characterization and Evaluation of Materials Analytical Chemistry Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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