NDLI: Highly sensitive electrochemical immunoassay for zearalenone in grain and grain-based food

Content Provider	Springer Nature Link
Author	Wang, Yuan Kai Yan, Ya Xian Mao, Zi Wei Wang, Heng an Zou, Qi Hao, Qian Wen Ji, Wen Hui Sun, Jian He
Copyright Year	2012
Abstract	We have developed a highly sensitive electrochemical immunoassay for the quantitation of zearalenone (ZEN), a mycotoxin produced by Fusarium species. In this enzyme linked immunosorbent assay, the enzymatic conversion of the substrate p-nitrophenylphosphate is detected by a microplate reader and the signal subsequently converted into an electrical signal. The concentrations of coating antigen (ZEN-ovalbumin), of monoclonal antibody, and of goat anti-mouse antibody labeled with alkaline phosphatase were optimized. In terms of electrochemical detection, the types and pH values of the buffers, the conditions for agitating, and scanning frequency were optimized. The effective detection range of this immunoassay is quite wide (0.004 to 9.5 ng mL−1), and the limit of detection is 2 pg mL−1. ZEN-free corn, wheat, and grain-based food samples were spiked with ZEN and analyzed by this method, and recoveries were found to range from 91.6 % to 113.0 %. Unlike previously described electrochemical methods, this method is both highly sensitive and has a wide working range. The method is fast and thus provides a platform for high-throughput analysis that meets the current need to monitor trace levels of analytes in grain and grain-based food. Figure Scheme of test procedure of electrochemical immunosensor (procedure of immune-reaction: from a to f)
Starting Page	187
Ending Page	193
Page Count	7
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	180
Issue Number	3-4
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2012-11-21
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Zearalenone Electrochemical analysis Immunoassay Highly sensitive Broad dynamic range Nanochemistry Nanotechnology Characterization and Evaluation of Materials Analytical Chemistry Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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