NDLI: Multiparametric diagnostics of cardiomyopathies by microRNA signatures

Content Provider	Springer Nature Link
Author	Siegismund, Christine S. Rohde, Maria Kühl, Uwe Lassner, Dirk
Copyright Year	2014
Abstract	The diagnosis of cardiomyopathies by endomyocardial biopsy analysis is the gold standard for confirmation of causative reasons but is failing if a sample does not contain the area of interest due to focal pathology. Biopsies are revealing an extract of the current situation of the heart muscle only, and the need for global organ-specific or systemic markers is obvious in order to minimize sampling errors. Global markers like specific gene expression signatures in myocardial tissue may therefore reflect the focal situation or condition of the whole myocardium. Besides gene expression profiles, microRNAs (miRNAs) represent a new group of stable biomarkers that are detectable both in tissue and body fluids. Such miRNAs may serve as cardiological biomarkers to characterize inflammatory processes, to confirm viral infections, and to differentiate various forms of infection. The predictive power of single miRNAs for diagnosis of complex diseases may be further increased if several distinctly deregulated candidates are combined to form a specific miRNA signature. Diagnostic systems that generate disease-related miRNA profiles are based on microarrays, bead-based oligo sorbent assays, or on assays based on real-time polymerase chain reactions and placed on microfluidic cards or nanowell plates. Multiparametric diagnostic systems that can measure differentially expressed miRNAs may become the diagnostic tool of the future due to their predictive value with respect to clinical course, therapeutic decisions, and therapy monitoring. We discuss here specific merits, limitations and the potential of currently available analytical platforms for diagnostics of heart muscle diseases based on miRNA profiling. Contains 34 references. Figure Modern diagnostics of cardiomyopathies will include multiparametric analysis of microRNA profiles in endomyocardial biopsies by real-time PCR or bead-based OLISA techniques. In contrast to high-throughput screening technologies diagnostic systems are realized by down-scaling sample volumes and simultaneous measurement of a limited number of stable disease related parameters.
Starting Page	1647
Ending Page	1653
Page Count	7
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	181
Issue Number	13-14
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2014-04-08
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biomarker Cardiomyopathy Endomyocardial biopsy MicroRNA Multiparametric analysis Nanochemistry Nanotechnology Characterization and Evaluation of Materials Analytical Chemistry Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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