NDLI: Activity of native hydrolytic enzymes and their association with the cell wall of three ectomycorrhizal fungi

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Activity of native hydrolytic enzymes and their association with the cell wall of three ectomycorrhizal fungi

Content Provider	Springer Nature Link
Author	Pérez de Mora, Alfredo Reuter, Bianca Lucio, Marianna Ahne, Alfred Schloter, Michael Pritsch, Karin
Copyright Year	2012
Abstract	The ecological and biogeochemical relevance of hydrolytic enzymes associated with the fungal cell wall has been poorly studied in ectomycorrhizal (ECM) fungi. We used a modified sequential extraction procedure to investigate the activity of various hydrolytic enzymes (β-glucosidase, acid-phosphatase, leucine-aminopeptidase, chitinase, xylanase and glucuronidase) and their association with the cell wall of three ECM fungi (Rhizopogon roseolus, Paxillus involutus and Piloderma croceum). Fungi were grown on C-rich solid medium under three different P concentrations (3.7, 0.37 and 0.037 mM). The sequential extraction procedure classifies enzymes as: (a) cytosolic, (b) loosely bound, (c) hydrophobically bound, (d) ionically bound and (e) covalently bound. Results showed that for the same fungus absolute enzymatic activity was affected by P concentration, whilst enzymatic compartmentalization among the cytosol and the cell wall fractions was not. The association of enzymes with the cell wall was fungus- and enzyme-specific. Our data indicate also that enzymes best known for being either extracellular or cytosolic or both, do act in muro as well. The ecological implications of cell wall-bound enzymes and the potential applications and limitations of sequential extractions are further discussed.
Starting Page	185
Ending Page	197
Page Count	13
File Format	PDF
ISSN	09406360
Journal	Mycorrhiza
Volume Number	23
Issue Number	3
e-ISSN	14321890
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-10-03
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cell wall-bound enzymes Paxillus involutus Piloderma croceum Rhizopogon roseolus Sequential extraction Multivariate analysis General linear model Microbiology Plant Sciences Ecology Agriculture Forestry
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Medicine Molecular Biology Ecology, Evolution, Behavior and Systematics

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